Protocols shared by authors via RaP service
Journal
Library construction and single-clone sequencing
Authors: Chong Zhang and Ming Shao
Date: Feb 1, 2022，Views：735
Original protocol used in research article：
Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish
Science Advances, Aug 5, 2021; DOI: 10.1126/sciadv.abg4243
ES and goods calculation
Authors: Raphaël A. Wittwer and Marcel G. A. van der Heijden
Date: Feb 1, 2022，Views：822
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
Data collection
Authors: Raphaël A. Wittwer and Marcel G. A. van der Heijden
Date: Feb 1, 2022，Views：745
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
Statistical analyses
Authors: Raphaël A. Wittwer and Marcel G. A. van der Heijden
Date: Feb 1, 2022，Views：729
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
Surgery and injection of viral vector
Authors: Christopher R Fetsch and Michael N Shadlen
Date: Jan 30, 2022，Views：777
Original protocol used in research article：
Focal optogenetic suppression in macaque area MT biases direction discrimination and decision confidence, but only transiently
eLife, Jul 27, 2018; DOI: 10.7554/eLife.36523
Detection of aptamer in tissues by qRT-PCR
Authors: Serena Zilio and Paolo Serafini
Date: Jan 23, 2022，Views：728
Original protocol used in research article：
Aptamers against mouse and human tumor-infiltrating myeloid cells as reagents for targeted chemotherapy
Science Translational Medicine, Jun 16, 2020; DOI: 10.1126/scitranslmed.aav9760
Histology and immunocytochemistry
Authors: Xuetao Sun and Sara S. Nunes
Date: Jan 21, 2022，Views：725
Original protocol used in research article：
Transplanted microvessels improve pluripotent stem cell–derived cardiomyocyte engraftment and cardiac function after infarction in rats
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aax2992
Ultraflexible substrates
Authors: Tsuyoshi Sekitani and Takafumi Uemura
Date: Jan 20, 2022，Views：690
Original protocol used in research article：
Imperceptible energy harvesting device and biomedical sensor based on ultraflexible ferroelectric transducers and organic diodes
Nature Communications, Apr 23, 2021; DOI: 10.1038/s41467-021-22663-6
Surgical procedures and viral vector injections
Authors: Kei Oyama, Yuji Nagai and Takafumi Minamimoto
Date: Jan 19, 2022，Views：772
Original protocol used in research article：
Chemogenetic dissection of the primate prefronto-subcortical pathways for working memory and decision-making
Science Advances, Jun 23, 2021; DOI: 10.1126/sciadv.abg4246
Cell cultures
Authors: Jack H. Brelstaff, Aviva M. Tolkovsky and Maria Grazia Spillantini
Date: Jan 15, 2022，Views：740
Original protocol used in research article：
Microglia become hypofunctional and release metalloproteases and tau seeds when phagocytosing live neurons with P301S tau aggregates
Science Advances, Oct 20, 2021; DOI: 10.1126/sciadv.abg4980
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