Bio-101 - Improve Research Reproducibility

Cloning and expression of SARS-CoV-2 RBD in the yeast Pichia pastoris

Authors: Mirko Paiardini, Maria Elena Bottazzi and Sudhir Pai Kasturi
Date: Dec 15, 2021，Views：828

Original protocol used in research article：

A yeast expressed RBD-based SARS-CoV-2 vaccine formulated with 3M-052-alum adjuvant promotes protective efficacy in non-human primates
Science Immunology, Jul 15, 2021; DOI: 10.1126/sciimmunol.abh3634

Study design

Authors: Manoj Kumar, Souvik Maiti and Debojyoti Chakraborty
Date: Dec 9, 2021，Views：872

Original protocol used in research article：

FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip
eLife, Jun 9, 2021; DOI: 10.7554/eLife.67130

Plasmid information

Authors: Xiuyi Song, Ruoming Xu and Tomoyasu Sugiyama
Date: Nov 29, 2021，Views：771

Original protocol used in research article：

Two plasmid modules for introducing the auxin-inducible degron into the fission yeast Schizosaccharomyces pombe by PCR-based gene targeting
microPublication Biology, Aug 18, 2021; DOI: 10.17912/micropub.biology.000442

TUNEL Assay

Author: Ella W. Englander
Date: Nov 19, 2021，Views：793

Original protocol used in research article：

Enhanced Antitumor Response to Immune Checkpoint Blockade Exerted by Cisplatin-Induced Mutagenesis in a Murine Melanoma Model
Frontiers in Oncology, Jul 6, 2021; DOI: 10.3389/fonc.2021.701968

Protocol for "Cryopreservation of dehydrated Caenorhabditis elegans with multiple recoveries using a granular medium"

Authors: Julia Hayden and Christopher Fang-Yen
Date: Nov 8, 2021，Views：743

Original protocol used in research article：

Cryopreservation of dehydrated Caenorhabditis elegans with multiple recoveries using a granular medium
microPublication Biology, Oct 25, 2021; DOI: 10.17912/micropub.biology.000488

Site-directed mutagenesis and vector construction

Author: Qing-Lin Liu
Date: Nov 7, 2021，Views：717

Original protocol used in research article：

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature
BMC Genomics, Jan 14, 2021; DOI: 10.1186/s12864-020-07365-5

Updated protocols for measurement of GSIS and calcium imaging of islet clusters

Authors: Jia Zhao, Shenghui Liang, Guang Gao, Liangyi Chen and Yanmei Liu
Date: Oct 31, 2021，Views：2213

Original protocol used in research article：

In vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development
eLife, Jan 29, 2019; DOI: 10.7554/eLife.41540

Purification of recombinant bacterial proteins

Authors: Min Young Kim and Chul Geun Kim
Date: Oct 31, 2021，Views：841

Original protocol used in research article：

Rational discovery of antimetastatic agents targeting the intrinsically disordered region of MBD2
Science Advances, Nov 19, 2019; DOI: 10.1126/sciadv.aav9810

Environmental Scanning Electron Microscopy (SEM)

Authors: Lucie Riglet, Isabelle Fobis-Loisy and Thierry Gaude
Date: Oct 14, 2021，Views：937

Original protocol used in research article：

KATANIN-dependent mechanical properties of the stigmatic cell wall mediate the pollen tube path in Arabidopsis
eLife, Sep 1, 2020; DOI: 10.7554/eLife.57282

MALDI-MS analysis

Authors: Yan Zhang, Joshua E Mayfield and Seema Irani
Date: Oct 11, 2021，Views：822

Original protocol used in research article：

Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
eLife, Aug 6, 2019; DOI: 10.7554/eLife.48725

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