Protocols shared by authors via RaP service
Journal
Manufacturing of hOBMT
Authors: Nathalie Bock, Dietmar W. Hutmacher and Judith A. Clements
Date: Oct 10, 2021，Views：856
Original protocol used in research article：
In vitro engineering of a bone metastases model allows for study of the effects of antiandrogen therapies in advanced prostate cancer
Science Advances, Jun 30, 2021; DOI: 10.1126/sciadv.abg2564
Multifunctionality assessments
Authors: Marcel G. A. van der Heijden and Raphaël Albert Wittwer
Date: Oct 6, 2021，Views：816
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
PARP1 Purification Protocol
Authors: Pam Dyer, Johannes Rudolph and Karolin Luger
Date: Sep 29, 2021，Views：949
Original protocol used in research article：
Poly(ADP-ribose) polymerase 1 searches DNA via a ‘monkey bar’ mechanism
eLife, Aug 8, 2018; DOI: 10.7554/eLife.37818
Headplate and cranial window surgery for two-photon imaging of inferior colliculus in transgenic mice
Authors: Aaron Benson Wong and J Gerard G Borst
Date: Sep 28, 2021，Views：958
Original protocol used in research article：
Tonotopic and non-auditory organization of the mouse dorsal inferior colliculus revealed by two-photon imaging
eLife, Oct 15, 2019; DOI: 10.7554/eLife.49091
Generation of transgenic lines
Authors: Wenqing Zhang and Jin Xu
Date: Sep 13, 2021，Views：885
Original protocol used in research article：
Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells
eLife, Jun 15, 2018; DOI: 10.7554/eLife.36131
Immunoprecipitation of recombinant LyzC with His-SUMO-Cab45 purified from HEK293 supernatant
Authors: Mai Ly Tran, Bulat R Ramazanov, Tobias Karl-Heinz Hecht and Julia von Blume
Date: Sep 1, 2021，Views：854
Original protocol used in research article：
Fam20C regulates protein secretion by Cab45 phosphorylation
The Journal of Cell Biology, May 18, 2020; DOI: 10.1083/jcb.201910089
Immunofluorescence Analysis of GLUT1 Expression
Authors: Sameer Mohammed, Saeed Al Mahri and Bahauddeen M. Alrfaei
Date: Aug 28, 2021，Views：789
Original protocol used in research article：
Proteomic and Molecular Assessment of the Common Saudi Variant in ACADVL Gene Through Mesenchymal Stem Cells
Frontiers in Cell and Developmental Biology, Jan 10, 2020; DOI: 10.3389/fcell.2019.00365
Cell clustering and classification
Author: James YH Li
Date: Aug 27, 2021，Views：812
Original protocol used in research article：
Specification of diverse cell types during early neurogenesis of the mouse cerebellum
eLife, Feb 8, 2019; DOI: 10.7554/eLife.42388
Track moving cells in 3D time lapse images by using 3DeeCellTracker
Authors: Chentao Wen and Koutarou D Kimura
Date: Aug 24, 2021，Views：1098
Original protocol used in research article：
3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images
eLife, Mar 30, 2021; DOI: 10.7554/eLife.59187
Colocalization analysis for immunostained zebrafish cryosections with or without label retention expansion microscopy (LR-ExM) by JACoP
Authors: Xiang Zhao and Su Guo
Date: Aug 11, 2021，Views：1006
Original protocol used in research article：
Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg1244
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