Protocols shared by authors via RaP service
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Immunoprecipitation of recombinant LyzC with His-SUMO-Cab45 purified from HEK293 supernatant
Authors: Mai Ly Tran, Bulat R Ramazanov, Tobias Karl-Heinz Hecht and Julia von Blume
Date: Sep 1, 2021，Views：856
Original protocol used in research article：
Fam20C regulates protein secretion by Cab45 phosphorylation
The Journal of Cell Biology, May 18, 2020; DOI: 10.1083/jcb.201910089
Immunofluorescence Analysis of GLUT1 Expression
Authors: Sameer Mohammed, Saeed Al Mahri and Bahauddeen M. Alrfaei
Date: Aug 28, 2021，Views：793
Original protocol used in research article：
Proteomic and Molecular Assessment of the Common Saudi Variant in ACADVL Gene Through Mesenchymal Stem Cells
Frontiers in Cell and Developmental Biology, Jan 10, 2020; DOI: 10.3389/fcell.2019.00365
Cell clustering and classification
Author: James YH Li
Date: Aug 27, 2021，Views：814
Original protocol used in research article：
Specification of diverse cell types during early neurogenesis of the mouse cerebellum
eLife, Feb 8, 2019; DOI: 10.7554/eLife.42388
Track moving cells in 3D time lapse images by using 3DeeCellTracker
Authors: Chentao Wen and Koutarou D Kimura
Date: Aug 24, 2021，Views：1100
Original protocol used in research article：
3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images
eLife, Mar 30, 2021; DOI: 10.7554/eLife.59187
Colocalization analysis for immunostained zebrafish cryosections with or without label retention expansion microscopy (LR-ExM) by JACoP
Authors: Xiang Zhao and Su Guo
Date: Aug 11, 2021，Views：1013
Original protocol used in research article：
Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg1244
Sample preparation TMT LC-MS3 mass spectrometry
Authors: Katherine A Donovan and Eric S Fischer
Date: Aug 5, 2021，Views：1016
Original protocol used in research article：
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
eLife, Aug 1, 2018; DOI: 10.7554/eLife.38430
Eicosanoid and cytokine analysis
Authors: Antonie Lechner and Julia Esser-von Bieren
Date: Aug 2, 2021，Views：798
Original protocol used in research article：
An anti-inflammatory eicosanoid switch mediates the suppression of type-2 inflammation by helminth larval products
Science Translational Medicine, Apr 21, 2020; DOI: 10.1126/scitranslmed.aay0605
Comet Assay for DNA Damage
Author: Jianmin Wan
Date: Jul 30, 2021，Views：904
Original protocol used in research article：
Earlier Degraded Tapetum1 (EDT1) Encodes an ATP-Citrate Lyase Required for Tapetum Programmed Cell Death1,[OPEN]
Plant Physiology, Sep 12, 2019; DOI: 10.1104/pp.19.00202
Image processing and data analysis
Authors: Daria Amiad-Pavlov, Dana Lorber and Talila Volk
Date: Jul 28, 2021，Views：855
Original protocol used in research article：
Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization
Science Advances, Jun 2, 2021; DOI: 10.1126/sciadv.abf6251
Video acquisition and CASA analysis of mouse sperm
Authors: Pei-Hsuan Wu, Zhiping Weng and Phillip D. Zamore
Date: Jul 27, 2021，Views：804
Original protocol used in research article：
The Evolutionarily Conserved piRNA-producing Locus pi6 Is Required for Male Mouse Fertility
Nat Genet, Jun 29, 2020; DOI: 10.1038/s41588-020-0657-7
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