Protocols shared by authors via RaP service
Journal
Detection of aptamer in tissues by qRT-PCR
Authors: Serena Zilio and Paolo Serafini
Date: Jan 23, 2022，Views：728
Original protocol used in research article：
Aptamers against mouse and human tumor-infiltrating myeloid cells as reagents for targeted chemotherapy
Science Translational Medicine, Jun 16, 2020; DOI: 10.1126/scitranslmed.aav9760
Histology and immunocytochemistry
Authors: Xuetao Sun and Sara S. Nunes
Date: Jan 21, 2022，Views：726
Original protocol used in research article：
Transplanted microvessels improve pluripotent stem cell–derived cardiomyocyte engraftment and cardiac function after infarction in rats
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aax2992
Ultraflexible substrates
Authors: Tsuyoshi Sekitani and Takafumi Uemura
Date: Jan 20, 2022，Views：694
Original protocol used in research article：
Imperceptible energy harvesting device and biomedical sensor based on ultraflexible ferroelectric transducers and organic diodes
Nature Communications, Apr 23, 2021; DOI: 10.1038/s41467-021-22663-6
Surgical procedures and viral vector injections
Authors: Kei Oyama, Yuji Nagai and Takafumi Minamimoto
Date: Jan 19, 2022，Views：777
Original protocol used in research article：
Chemogenetic dissection of the primate prefronto-subcortical pathways for working memory and decision-making
Science Advances, Jun 23, 2021; DOI: 10.1126/sciadv.abg4246
Cell cultures
Authors: Jack H. Brelstaff, Aviva M. Tolkovsky and Maria Grazia Spillantini
Date: Jan 15, 2022，Views：743
Original protocol used in research article：
Microglia become hypofunctional and release metalloproteases and tau seeds when phagocytosing live neurons with P301S tau aggregates
Science Advances, Oct 20, 2021; DOI: 10.1126/sciadv.abg4980
Isolation of EVs from brain parenchyma
Authors: Pasquale D’Acunzo and Efrat Levy
Date: Jan 14, 2022，Views：757
Original protocol used in research article：
Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in Down syndrome
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abe5085
TE and enhancer annotations
Author: Miguel R Branco
Date: Jan 14, 2022，Views：884
Original protocol used in research article：
Functional evaluation of transposable elements as enhancers in mouse embryonic and trophoblast stem cells
eLife, Apr 23, 2019; DOI: 10.7554/eLife.44344
3D nanoendoscopy-AFM
Authors: Marcos Penedo, Keisuke Miyazawa, Naoko Okano, Hirotoshi Furusho, Takehiko Ichikawa, Mohammad Shahidul Alam, Kazuki Miyata, Chikashi Nakamura and Takeshi Fukuma
Date: Jan 13, 2022，Views：686
Original protocol used in research article：
Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abj4990
Calculating the relative abundances of STs from metagenomic data
Authors: Jay-Hyun Jo and Heidi H. Kong
Date: Jan 12, 2022，Views：713
Original protocol used in research article：
Alterations of human skin microbiome and expansion of antimicrobial resistance after systemic antibiotics
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abd8077
scRNA-Seq data generation
Authors: Ranran Gao, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：946
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
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