Protocols shared by authors via RaP service
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Identification of individual potentially tumor-reactive T cells
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：782
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Indexes characterizing meta-clusters and STARTRAC analysis
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：927
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Immunohistochemistry
Authors: Jose Felix Moruno Manchon and Andrey S Tsvetkov
Date: Mar 3, 2022，Views：655
Original protocol used in research article：
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI: 10.7554/eLife.52283
Cell cultures and transfection
Authors: Jose Felix Moruno Manchon and Andrey S Tsvetkov
Date: Mar 3, 2022，Views：653
Original protocol used in research article：
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI: 10.7554/eLife.52283
DiI tracing
Authors: Nicolas Renier and Alain Chédotal
Date: Mar 3, 2022，Views：647
Original protocol used in research article：
A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
eLife, Mar 28, 2017; DOI: 10.7554/eLife.23494
Selection of genetic instruments
Authors: Wuqing Huang, Jun Xiao, Jianguang Ji and Liangwan Chen
Date: Mar 2, 2022，Views：730
Original protocol used in research article：
Association of lipid-lowering drugs with COVID-19 outcomes from a Mendelian randomization study
eLife, Dec 6, 2021; DOI: 10.7554/eLife.73873
Microinjection and selection and confirmation of transgenic zebrafish
Author: Chiou-Hwa Yuh
Date: Mar 2, 2022，Views：591
Original protocol used in research article：
l-Carnitine ameliorates congenital myopathy in a tropomyosin 3 de novo mutation transgenic zebrafish
Journal of Biomedical Science, Jan 12, 2021; DOI: 10.1186/s12929-020-00707-1
Cell culture, transfection, and drug treatments
Authors: Jason E. Lee and Gia K. Voeltz
Date: Mar 2, 2022，Views：634
Original protocol used in research article：
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108
Cell activation and immunostaining
Author: Jesse Goyette
Date: Mar 2, 2022，Views：661
Original protocol used in research article：
Protein-PAINT: Superresolution microscopy with signaling proteins
Science Signaling, Feb 1, 2022; DOI: 10.1126/scisignal.abg9782
Conjunction of DNA and primary antibody
Authors: Xiaohao Liu, Fenyong Sun, Yu Yang, Xiaoli Zhu and Weihong Tan
Date: Mar 2, 2022，Views：708
Original protocol used in research article：
Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging
Science Advances, Jan 14, 2022; DOI: 10.1126/sciadv.abk0133
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