Protocols shared by authors via RaP service
Journal
Cell culture
Authors: Xiaohao Liu, Fenyong Sun, Yu Yang, Xiaoli Zhu and Weihong Tan
Date: Apr 8, 2022，Views：648
Original protocol used in research article：
Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging
Science Advances, Jan 14, 2022; DOI: 10.1126/sciadv.abk0133
Computational model
Authors: Satoshi Toda, Wendell A. Lim and Teemu J. Hakkinen
Date: Apr 7, 2022，Views：656
Original protocol used in research article：
Engineering synthetic morphogen systems that can program multicellular patterning
Science, Oct 15, 2020; DOI: 10.1126/science.abc0033
Lentiviral
Authors: Kotryna Vaidziulyte, Kristine Schauer and Mathieu Coppey
Date: Apr 6, 2022，Views：801
Original protocol used in research article：
Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking
eLife, Mar 18, 2022; DOI: 10.7554/eLife.69229
Northern tRNA charging assay
Authors: Natalya N Pavlova and Craig B Thompson
Date: Apr 1, 2022，Views：722
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
Histone extraction and derivatization
Authors: Sydney P Thomas and John M Denu
Date: Apr 1, 2022，Views：760
Original protocol used in research article：
Short-chain fatty acids activate acetyltransferase p300
eLife, Oct 22, 2021; DOI: 10.7554/eLife.72171
2.1. X-ITE Pain Database
Author: Ehsan Othman
Date: Apr 1, 2022，Views：705
Original protocol used in research article：
Automatic vs. Human Recognition of Pain Intensity from Facial Expression on the X-ITE Pain Database
Sensors (Basel, Switzerland), May 10, 2021; DOI: 10.3390/s21093273
Deconvolution analysis
Authors: Tao Wei, Jihwan Park, Heng Xu, Han Luo and Gyeong Dae Kim
Date: Mar 28, 2022，Views：629
Original protocol used in research article：
Characterizing dedifferentiation of thyroid cancer by integrated analysis
Science Advances, Jul 27, 2021; DOI: 10.1126/sciadv.abf3657
Data Analysis
Authors: Tianying Zhong and Yun Gu
Date: Mar 27, 2022，Views：591
Original protocol used in research article：
Label-Free Quantitative Comparison of Cervical Mucus Peptides in Subjects With Endocervical Adenocarcinoma and Adenocarcinoma in Situ
Technology in Cancer Research & Treatment, Feb 22, 2021; DOI: 10.1177/1533033821997825
Slingshot
Authors: Bryan Marsh and Robert Blelloch
Date: Mar 24, 2022，Views：672
Original protocol used in research article：
Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266
Generation of E. coli strains
Authors: Shar-yin N. Huang, Nadim Majdalani and Yves Pommier
Date: Mar 22, 2022，Views：689
Original protocol used in research article：
Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes
Science Advances, Mar 3, 2021; DOI: 10.1126/sciadv.abe0384
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