Protocols shared by authors via RaP service
Journal
JAGuaR Alignment
Author: Richard Corbett
Date: Apr 26, 2022，Views：593
Original protocol used in research article：
A Scalable Strand-Specific Protocol Enabling Full-Length Total RNA Sequencing From Single Cells
Frontiers in Genetics, Jun 3, 2021; DOI: 10.3389/fgene.2021.665888
Preparation of bovine serum albumin (BSA)-conjugated palmitic acid
Author: Fei Hua
Date: Apr 26, 2022，Views：943
Original protocol used in research article：
Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway
BMC Nephrology, Jun 10, 2021; DOI: 10.1186/s12882-021-02391-1
Proliferation assay and cell cycle profiling via flow cytometry
Author: Li Lan
Date: Apr 26, 2022，Views：616
Original protocol used in research article：
cGAS suppresses genomic instability as a decelerator of replication forks
Science Advances, Oct 13, 2020; DOI: 10.1126/sciadv.abb8941
Immunohistochemistry
Author: Sungjin Park
Date: Apr 25, 2022，Views：616
Original protocol used in research article：
The release of surface-anchored α-tectorin an apical extracellular matrix protein mediates tectorial membrane organization
Science Advances, Nov 26, 2019; DOI: 10.1126/sciadv.aay6300
Coevolutionary analysis and coarse-grained simulations
Authors: Francesco Colizzi and Modesto Orozco
Date: Apr 25, 2022，Views：613
Original protocol used in research article：
Probing allosteric regulations with coevolution-driven molecular simulations
Science Advances, Sep 8, 2021; DOI: 10.1126/sciadv.abj0786
Single-cell TCR sequencing
Authors: Daniel T. Leung and Owen Jensen
Date: Apr 22, 2022，Views：634
Original protocol used in research article：
A subset of follicular helper-like MAIT cells can provide B cell help and support antibody production in the mucosa
Science Immunology, Jan 14, 2022; DOI: 10.1126/sciimmunol.abe8931
mAb competition assay
Authors: Tomas Strandin, Andrew S. Herbert, Mattias N. E. Forsell, Laura M. Walker and Kartik Chandran
Date: Apr 21, 2022，Views：579
Original protocol used in research article：
Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
Science Translational Medicine, Mar 16, 2022; DOI: 10.1126/scitranslmed.abl5399
Differential gene expression analysis
Authors: Wei Xie, Xiaotong Wu and Hongmei Zhang
Date: Apr 21, 2022，Views：632
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Statistical analysis
Authors: Ramona A.J. Zwamborn and Jan H. Veldink
Date: Apr 20, 2022，Views：607
Original protocol used in research article：
Genome-wide study of DNA methylation shows alterations in metabolic, inflammatory, and cholesterol pathways in ALS
Science Translational Medicine, Feb 23, 2022; DOI: 10.1126/scitranslmed.abj0264
Induction of rEpiLCs and rPGCLCs
Authors: Mami Oikawa, Masumi Hirabayashi and Toshihiro Kobayashi
Date: Apr 19, 2022，Views：627
Original protocol used in research article：
Functional primordial germ cell–like cells from pluripotent stem cells in rats
Science, Apr 7, 2022; DOI: 10.1126/science.abl4412
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