Protocols shared by authors via RaP service
Journal
Generation of SH-SY5Y HTT knockout cell lines by CRISPR-Cas9
Author: Jianning Wei
Date: Jul 13, 2023，Views：967
Original protocol used in research article：
RNA-seq analysis reveals significant transcriptome changes in huntingtin-null human neuroblastoma cells
BMC Medical Genomics, Jul 2, 2021; DOI: 10.1186/s12920-021-01022-w
HepaRG culture
Author: Francesco Bellanti
Date: Jul 13, 2023，Views：874
Original protocol used in research article：
Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiation
NPJ Regenerative Medicine, May 26, 2021; DOI: 10.1038/s41536-021-00137-z
MAG Bead DNA Extraction Protocol
Authors: Tamara D. Simon and Christopher E. Pope
Date: Jul 11, 2023，Views：730
Original protocol used in research article：
Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643
CyTOF analysis
Author: Chu-Xia Deng
Date: Jul 11, 2023，Views：726
Original protocol used in research article：
Cullin-5 deficiency orchestrates the tumor microenvironment to promote mammary tumor development through CREB1-CCL2 signaling
Science Advances, Jan 20, 2023; DOI: 10.1126/sciadv.abq1395
Analysis of differential expression in tumor and matched normal tissues
Author: Ying Yuan
Date: Jul 1, 2023，Views：746
Original protocol used in research article：
Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer
Chinese Journal of Cancer Research, Feb 28, 2021; DOI: 10.21147/j.issn.1000-9604.2021.01.10
Immunofluorescence, immunoprecipitation, and ELISA using the Scicons anti-dsRNA J2 antibody
Authors: Elizabeth Ochoa and Bess Frost
Date: Jun 22, 2023，Views：961
Original protocol used in research article：
Pathogenic tau–induced transposable element–derived dsRNA drives neuroinflammation
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq5423
APEX2 biotinylation in brain slices
Authors: David Sulzer and Peter A Sims
Date: Jun 21, 2023，Views：948
Original protocol used in research article：
Subcellular proteomics of dopamine neurons in the mouse brain
eLife, Jan 31, 2022; DOI: 10.7554/eLife.70921
CUT&RUN library preparation
Author: Paolo Emanuele Forni
Date: Jun 20, 2023，Views：55
Original protocol used in research article：
Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons
eLife, ; DOI: 10.7554/eLife.77259
Sortase A conjugation of HIV-1 envelope trimers to ferritin nanoparticles
Author: Kevin O. Saunders
Date: Jun 13, 2023，Views：923
Original protocol used in research article：
Targeted selection of HIV-specific antibody mutations by engineering B cell maturation
Science, Dec 5, 2019; DOI: 10.1126/science.aay7199
crystal fish imaging
Authors: Francois Kroll and Jason Rihel
Date: Jun 13, 2023，Views：881
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.