Protocols shared by authors via RaP service
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Quantitative real-time polymerase chain reaction (qRT-PCR)
Authors: Yong-Hyun Han and Gwendalyn J. Randolph
Date: Sep 4, 2023，Views：125
Original protocol used in research article：
Enterically derived high-density lipoprotein restrains liver injury through the portal vein
Science, Jul 23, 2021; DOI: 10.1126/science.abe6729
RNase A treatment
Authors: Niladri K Sinha, Roland Beckmann and Rachel Green
Date: Sep 1, 2023，Views：187
Original protocol used in research article：
EDF1 coordinates cellular responses to ribosome collisions
eLife, Aug 3, 2020; DOI: 10.7554/eLife.58828
Isolation of synaptosomes and postsynaptic densities
Authors: M. Neal Waxham and Ilya Levental
Date: Sep 1, 2023，Views：243
Original protocol used in research article：
Remodeling of the postsynaptic plasma membrane during neural development
Molecular Biology of the Cell, Nov 7, 2016; DOI: 10.1091/mbc.e16-06-0420
TF transposon genotyping PCRs for granulosa differentiation
Authors: Merrick D Pierson Smela and George M Church
Date: Sep 1, 2023，Views：49
Original protocol used in research article：
Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression
eLife, Feb 21, 2023; DOI: 10.7554/eLife.83291
Animal experiments and echocardiography assessment
Authors: Dingli Xu and Qingchun Zeng
Date: Sep 1, 2023，Views：173
Original protocol used in research article：
Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis
Science Advances, Jun 2, 2023; DOI: 10.1126/sciadv.adg0478
Interpretation of Fractional Inhibitory Concentration Index (FICI)
Authors: Wenli Feng and Jing Yang
Date: Sep 1, 2023，Views：827
Original protocol used in research article：
Cotreatment with Aspirin and Azole Drugs Increases Sensitivity of Candida albicans in vitro
Infect Drug Resist, Jun 2, 2021; DOI: 10.2147/IDR.S314538
Quantitative polymerase chain reaction (qPCR)
Authors: Ke-Hsuan Wei, Shih-Lei (Ben) Lai and I-Ting Lin
Date: Aug 31, 2023，Views：182
Original protocol used in research article：
Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration
eLife, Jul 27, 2023; DOI: 10.7554/eLife.84679
Cytokine release assay
Authors: Vanaja Konduri and William K. Decker
Date: Aug 31, 2023，Views：198
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
2.8. Mitochondrial membrane potential (ΔΨm) and mtROS
Authors: Sehwan Jang and Sabzali Javadov
Date: Aug 31, 2023，Views：204
Original protocol used in research article：
Elucidating the contribution of mitochondrial glutathione to ferroptosis in cardiomyocytes
Redox Biology, Jun 1, 2021; DOI: 10.1016/j.redox.2021.102021
Tryptophan-quenching experiments
Author: Krishnananda Chattopadhyay
Date: Aug 29, 2023，Views：236
Original protocol used in research article：
The metal cofactor zinc and interacting membranes modulate SOD1 conformation-aggregation landscape in an in vitro ALS model
eLife, Apr 7, 2021; DOI: 10.7554/eLife.61453
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