Protocols shared by authors via RaP service
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Detailed BrdU immuno protocol with anti-YFP pre-staining
Authors: Zoe Christenson Wick, Esther Krook-Magnuson and Madison Tetzlaff
Date: Jan 27, 2020，Views：1058
Original protocol used in research article：
Novel long-range inhibitory nNOS-expressing hippocampal cells
eLife, Oct 14, 2019; DOI: 10.7554/eLife.46816
Pull-down and identification of the ligand
Author: Timo Vögtle
Date: Jan 23, 2020，Views：1276
Original protocol used in research article：
Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
eLife, Aug 22, 2019; DOI: 10.7554/eLife.46840
MWC model simulations
Authors: Olga Rapp and Ofer Yifrach
Date: Jan 20, 2020，Views：1193
Original protocol used in research article：
Evolutionary and functional insights into the mechanism underlying body-size-related adaptation of mammalian hemoglobin
eLife, Oct 24, 2019; DOI: 10.7554/eLife.47640
Immunohistochemical analysis of early passage cultures
Author: Krishna Kalyan Kolluri
Date: Jan 14, 2020，Views：1302
Original protocol used in research article：
Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells
eLife, Jan 18, 2018; DOI: 10.7554/eLife.30224
Chromatin and whole cell Flow cytometry
Authors: Jacob Peter Matson and Jeanette Gowen Cook
Date: Jan 14, 2020，Views：1286
Original protocol used in research article：
Rapid DNA replication origin licensing protects stem cell pluripotency
eLife, Nov 17, 2017; DOI: 10.7554/eLife.30473
Gene ontology analysis, expression analysis and SNP functional annotation
Authors: Fan Liu and Ziyi Xiong
Date: Jan 13, 2020，Views：1549
Original protocol used in research article：
Novel genetic loci affecting facial shape variation in humans
eLife, Nov 26, 2019; DOI: 10.7554/eLife.49898
iDISCO+ processing and light-sheet microscopy
Author: Nicolas Renier
Date: Jan 9, 2020，Views：1253
Original protocol used in research article：
A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
eLife, Mar 28, 2017; DOI: 10.7554/eLife.23494
Organotypic hippocampal slice cultures – electrophysiology and random-access two-photon voltage imaging
Author: Katalin Toth
Date: Jan 9, 2020，Views：1398
Original protocol used in research article：
Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators
eLife, Jul 27, 2017; DOI: 10.7554/eLife.25690
Quantitative image analysis
Author: Charlotte Kirchhelle
Date: Jan 9, 2020，Views：1078
Original protocol used in research article：
Two mechanisms regulate directional cell growth in Arabidopsis lateral roots
eLife, Jul 29, 2019; DOI: 10.7554/eLife.47988
Quantification of phenotypes
Author: Zhe Chen
Date: Jan 7, 2020，Views：1028
Original protocol used in research article：
Temporal regulation of axonal repulsion by alternative splicing of a conserved microexon in mammalian Robo1 and Robo2
eLife, Aug 8, 2019; DOI: 10.7554/eLife.46042
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