Protocols shared by authors via RaP service
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SNARE constructs
Authors: Junyi Jiao, Frederick M Hughson and Yongli Zhang
Date: Mar 5, 2020，Views：1106
Original protocol used in research article：
Munc18-1 catalyzes neuronal SNARE assembly by templating SNARE association
eLife, Dec 12, 2018; DOI: 10.7554/eLife.41771
Tagmentation procedure for ATAC-seq of sorted GFP+ endothelial cells
Authors: Mark F Sabbagh and Jeremy Nathans
Date: Mar 4, 2020，Views：1551
Original protocol used in research article：
A genome-wide view of the de-differentiation of central nervous system endothelial cells in culture
eLife, Jan 8, 2020; DOI: 10.7554/eLife.51276
Assessment of mortality
Author: Xia Li
Date: Mar 4, 2020，Views：1285
Original protocol used in research article：
Longitudinal trajectories, correlations and mortality associations of nine biological ages across 20-years follow-up
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51507
Fly strains
Authors: Fengwei Yu and menglong Rui
Date: Mar 3, 2020，Views：1134
Original protocol used in research article：
Patronin governs minus-end-out orientation of dendritic microtubules to promote dendrite pruning in Drosophila
eLife, Mar 28, 2019; DOI: 10.7554/eLife.39964
Genome editing by CRISPR-Cas9
Authors: Rui Jia and Juan S Bonifacino
Date: Mar 2, 2020，Views：1225
Original protocol used in research article：
Negative regulation of autophagy by UBA6-BIRC6–mediated ubiquitination of LC3
eLife, Nov 6, 2019; DOI: 10.7554/eLife.50034
Construction of the RapiDeg yeast strain
Author: Lu Zhu
Date: Feb 26, 2020，Views：1151
Original protocol used in research article：
ESCRTs function directly on the lysosome membrane to downregulate ubiquitinated lysosomal membrane proteins
eLife, Jun 29, 2017; DOI: 10.7554/eLife.26403
Bayesian inference of progenitor types
Authors: Alfredo Llorca and Oscar Marin
Date: Feb 26, 2020，Views：1115
Original protocol used in research article：
A stochastic framework of neurogenesis underlies the assembly of neocortical cytoarchitecture
eLife, Nov 18, 2019; DOI: 10.7554/eLife.51381
Colony formation assay
Author: Ralph Patrick
Date: Feb 25, 2020，Views：4295
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
Genome Editing
Author: Grant Parker Flowers
Date: Feb 25, 2020，Views：1208
Original protocol used in research article：
Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls
eLife, Jan 28, 2020; DOI: 10.7554/eLife.48511
Single cell isolation from SVF
Author: Prashant Rajbhandari
Date: Feb 25, 2020，Views：1435
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
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