Protocols shared by authors via RaP service
Journal
Single-cell targeted mRNA and antibody sequencing
Author: Z. Zhang
Date: Mar 31, 2020，Views：1023
Original protocol used in research article：
Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aaz0374
Genetic labeling and pre- and post-sequencing quality checks of SGNs, HCs, and glial cells, and library construction
Author: Zhiyong Liu
Date: Mar 31, 2020，Views：1291
Original protocol used in research article：
Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages
eLife, Jan 8, 2020; DOI: 10.7554/eLife.50491
Protocol for measuring plasma membrane:cytoplasm fluorescence intensity ratios
Authors: Lisa M. Grove and Mitchell A. Olman
Date: Mar 27, 2020，Views：920
Original protocol used in research article：
Translocation of TRPV4-PI3Kγ complexes to the plasma membrane drives myofibroblast transdifferentiation
Science Signaling, Nov 11, 2019; DOI: 10.1126/scisignal.aau1533
Development of the aspiration-assisted bioprinter
Author: Ibrahim T. Ozbolat
Date: Mar 25, 2020，Views：979
Original protocol used in research article：
Aspiration-assisted bioprinting for precise positioning of biologics
Science Advances, Mar 5, 2020; DOI: 10.1126/sciadv.aaw5111
Cell culture
Authors: Vladimir I Gelfand and Rosalind Norkett
Date: Mar 20, 2020，Views：1026
Original protocol used in research article：
Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding
eLife, Feb 5, 2020; DOI: 10.7554/eLife.52009
Sample preparation for fPALM imaging
Authors: Nathan A McDonald and Kathleen L Gould
Date: Mar 17, 2020，Views：1017
Original protocol used in research article：
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring
eLife, Sep 15, 2017; DOI: 10.7554/eLife.28865
Measurement of acetyl-CoA
Author: Pamela Maher
Date: Mar 17, 2020，Views：1451
Original protocol used in research article：
Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866
Isolation and cell culture of primary alveolar macrophages
Authors: Einar Kleinhans Krogsaeter, Barbara Spix, Elisabeth Butz, Anna Scotto Rosato and Christian Grimm
Date: Mar 17, 2020，Views：1765
Original protocol used in research article：
Selective agonist of TRPML2 reveals direct role in chemokine release from innate immune cells
eLife, Nov 27, 2018; DOI: 10.7554/eLife.39720
Integration method (with replacement of go omissions)
Author: Frederick Verbruggen
Date: Mar 17, 2020，Views：1652
Original protocol used in research article：
A consensus guide to capturing the ability to inhibit actions and impulsive behaviors in the stop-signal task
eLife, Apr 29, 2019; DOI: 10.7554/eLife.46323
Immunofluorescence
Author: Nicolas Damond
Date: Mar 17, 2020，Views：1065
Original protocol used in research article：
Blockade of glucagon signaling prevents or reverses diabetes onset only if residual β-cells persist
eLife, Apr 19, 2016; DOI: 10.7554/eLife.13828
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