Protocols shared by authors via RaP service
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RNA seq data analysis
Author: Ilaria Granata
Date: Apr 15, 2020，Views：1124
Original protocol used in research article：
Human axial progenitors generate trunk neural crest cells in vitro
eLife, Aug 10, 2018; DOI: 10.7554/eLife.35786
Immunofluorescent staining of mouse embryonic tissues
Authors: Jingyue Xu and Rulang Jiang
Date: Apr 14, 2020，Views：994
Original protocol used in research article：
Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
eLife, Jan 14, 2019; DOI: 10.7554/eLife.40315
Purification of APP C100-FLAG substrate
Authors: David M Bolduc, Michael S Wolfe and Dennis J Selkoe
Date: Apr 14, 2020，Views：1194
Original protocol used in research article：
The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase
eLife, Aug 31, 2016; DOI: 10.7554/eLife.17578
Step-by-step lamina propria cell isolation protocol for H. polygyrus infected intestines
Author: Johannes U Mayer
Date: Apr 13, 2020，Views：1423
Original protocol used in research article：
High-dimensional analysis of intestinal immune cells during helminth infection
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51678
β-Arrestin recruitment
Authors: Alix A. J. Rouault and Julien A. Sebag
Date: Apr 13, 2020，Views：1138
Original protocol used in research article：
The GPCR accessory protein MRAP2 regulates both biased signaling and constitutive activity of the ghrelin receptor GHSR1a
Science Signaling, Jan 6, 2020; DOI: 10.1126/scisignal.aax4569
Whole mount immunostaining
Author: Manju Sharma
Date: Apr 13, 2020，Views：1268
Original protocol used in research article：
Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF
eLife, May 31, 2019; DOI: 10.7554/eLife.43352
Cell surface ELISA
Author: Amanda N Hayward
Date: Apr 13, 2020，Views：1436
Original protocol used in research article：
A toolkit for studying cell surface shedding of diverse transmembrane receptors
eLife, Jun 7, 2019; DOI: 10.7554/eLife.46983
RNP assembly for HDR reaction
Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1222
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Expression and purification of azido-Cas9 mutants
Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1260
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Bacterial culturing
Authors: Lina Yao and A Sloan Devlin
Date: Apr 11, 2020，Views：1155
Original protocol used in research article：
A selective gut bacterial bile salt hydrolase alters host metabolism
eLife, Jul 17, 2018; DOI: 10.7554/eLife.37182
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