Bio-101 - Improve Research Reproducibility

Immunofluorescent labelling of adult mouse neuromuscular junctions

Authors: Roberta Sartori, Adam Hagg, Paul Gregorevic and Marco Sandri
Date: Dec 7, 2023，Views：2270

Original protocol used in research article：

Perturbed BMP signaling and denervation promote muscle wasting in cancer cachexia
Science Translational Medicine, Aug 4, 2021; DOI: 10.1126/scitranslmed.aay9592

Mouse Aortic Ring Assay

Authors: Carmen Ruiz de Almodovar and Xiaohong Wang
Date: Dec 5, 2023，Views：2521

Original protocol used in research article：

Protein Phosphatase 2A Mediates YAP Activation in Endothelial Cells Upon VEGF Stimulation and Matrix Stiffness
Frontiers in Cell and Developmental Biology, May 13, 2021; DOI: 10.3389/fcell.2021.675562

Myelin phagocytosis and clearance assays

Author: Rasmus Berglund
Date: Dec 4, 2023，Views：2540

Original protocol used in research article：

Microglial autophagy–associated phagocytosis is essential for recovery from neuroinflammation
Science Immunology, Oct 15, 2020; DOI: 10.1126/sciimmunol.abb5077

TeSLA for TL measurement

Author: Jerry W. Shay
Date: Dec 4, 2023，Views：2288

Original protocol used in research article：

A method for measuring the distribution of the shortest telomeres in cells and tissues
Nature Communications, Nov 7, 2017; DOI: 10.1038/s41467-017-01291-z

CRISPR/Cas9-mediated gene editing

Authors: Alyssa N. Werner and Pascale G. Charest
Date: Nov 29, 2023，Views：2554

Original protocol used in research article：

CRISPR-mediated reversion of oncogenic KRAS mutation results in increased proliferation and reveals independent roles of Ras and mTORC2 in the migration of A549 lung cancer cells
Molecular Biology of the Cell, Sep 20, 2023; DOI: 10.1091/mbc.E23-05-0152

Plasmid map (pGD009)

Authors: Guillaume Diss and Ben Lehner
Date: Nov 27, 2023，Views：43

Original protocol used in research article：

The genetic landscape of a physical interaction
eLife, Apr 11, 2018; DOI: 10.7554/eLife.32472

Association Analysis

Authors: Saba Naz and Vinay Kumar Nandicoori
Date: Nov 23, 2023，Views：2713

Original protocol used in research article：

GWAS and functional studies suggest a role for altered DNA repair in the evolution of drug resistance in Mycobacterium tuberculosis
eLife, Jan 25, 2023; DOI: 10.7554/eLife.75860

Protein expression in bacteria and purification

Authors: Huili Zhang, Feifei Yu, Yidan Ouyang and Qi Xie
Date: Nov 23, 2023，Views：2484

Original protocol used in research article：

A Gγ protein regulates alkaline sensitivity in crops
Science, Mar 24, 2023; DOI: 10.1126/science.ade8416

PRS for LOY in UKB

Author: Nicholas C. P. Cross
Date: Nov 21, 2023，Views：17

Original protocol used in research article：

Age-related loss of chromosome Y is associated with levels of sex hormone binding globulin and clonal hematopoiesis defined by TET2, TP53, and CBL mutations
Science Advances, Apr 21, 2023; DOI: 10.1126/sciadv.ade9746

NanoBiT Luciferase-based live-cell assay for cell–cell fusion quantification

Authors: Kryštof Štafl, Martin Trávníček, Jiří Hejnar and Kateřina Trejbalová
Date: Nov 21, 2023，Views：2654

Original protocol used in research article：

Heterologous avian system for quantitative analysis of Syncytin-1 interaction with ASCT2 receptor
Retrovirology, Jun 22, 2021; DOI: 10.1186/s12977-021-00558-0

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