Bio-101 - Improve Research Reproducibility

Protocol for Determining RNA Localization by Subcellular RNA Fractionation

Authors: Irfana Saleem, Bhopal Mohapatra, Hamid Band and Vimla Band
Date: Apr 22, 2024，Views：824

Original protocol used in research article：

The Mammalian Ecdysoneless Protein Interacts with RNA Helicase DDX39A To Regulate Nuclear mRNA Export
Molecular and Cellular Biology, Jun 23, 2021; DOI: 10.1128/mcb.00103-21

In vivo experiments in humanized Alb−/−FcRn−/−hFcRn+/+ mice

Author: Joan Clària
Date: Apr 22, 2024，Views：18

Original protocol used in research article：

Albumin internalizes and inhibits endosomal TLR signaling in leukocytes from patients with decompensated cirrhosis
Science Translational Medicine, Oct 20, 2020; DOI: 10.1126/scitranslmed.aax5135

In-gel fluorescence imaging and immunoblotting

Authors: Hayashi Yamamoto and Noboru Mizushima
Date: Apr 19, 2024，Views：903

Original protocol used in research article：

A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
eLife, ; DOI: 10.7554/eLife.78923

Lead contact

Authors: Wen Fang and Liping Wang
Date: Apr 18, 2024，Views：110

Original protocol used in research article：

Neural dynamics of causal inference in the macaque frontoparietal circuit
eLife, Oct 24, 2022; DOI: 10.7554/eLife.76145

Cryo-Sectioning of viral tracer-labeled macaque brains

Authors: Mingchao Yan and Zheng Wang
Date: Apr 15, 2024，Views：821

Original protocol used in research article：

Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution and comparison with diffusion tractography
eLife, ; DOI: 10.7554/eLife.72534

Protocol for preparation of Quantum dot loaded dissolvable microneedle patches

Authors: Ana Jaklenec, Robert Langer and Kevin J. McHugh
Date: Apr 11, 2024，Views：977

Original protocol used in research article：

Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination
Science Translational Medicine, Dec 17, 2019; DOI: 10.1126/scitranslmed.aay7162

Stimulus sets in the study entitled 'Emerged human-like facial expression representation in a deep convolutional neural network'

Authors: Liqin Zhou, Ming Meng, Ke Zhou and Anmin Yang
Date: Apr 10, 2024，Views：28

Original protocol used in research article：

Emerged human-like facial expression representation in a deep convolutional neural network
Science Advances, Mar 23, 2022; DOI: 10.1126/sciadv.abj4383

Quantitative assessment of Stat92E-GFP expression and anti–p-Stat staining during oogenesis

Authors: Jhen-Wei Wu, Yu-Chiuan Chang and Anna C.-C. Jang
Date: Apr 1, 2024，Views：15

Original protocol used in research article：

Spatiotemporal gating of Stat nuclear influx by Drosophila Npas4 in collective cell migration
Science Advances, Jul 22, 2022; DOI: 10.1126/sciadv.abm2411

Isolation of Lamina Propria Cells from Mouse Small and Large Intestines

Authors: Qing Zhao and Charles O. Elson
Date: Mar 29, 2024，Views：738

Original protocol used in research article：

CD4+ T cell activation and concomitant mTOR metabolic inhibition can ablate microbiota-specific memory cells and prevent colitis
Science Immunology, Dec 11, 2020; DOI: 10.1126/sciimmunol.abc6373

Sulfate detection by ion-exchange chromatography

Authors: Maria Dalgaard Mikkelsen and Anne S. Meyer
Date: Mar 29, 2024，Views：26

Original protocol used in research article：

A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure
Scientific Reports, Sep 30, 2021; DOI: 10.1038/s41598-021-98588-3

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