Bio-101 - Improve Research Reproducibility

Single-Cell TCR Sequencing

Authors: Bernardo S. Reis and Daniel Mucida
Date: Dec 19, 2023，Views：2237

Original protocol used in research article：

TCR-Vγδ usage distinguishes protumor from antitumor intestinal γδ T cell subsets
Science, Jul 14, 2022; DOI: 10.1126/science.abj8695

IL-2/JES6-1 and IL-2/S4B6 complexes

Author: Marek Kovar
Date: Dec 19, 2023，Views：2272

Original protocol used in research article：

IL-2/JES6-1 mAb complexes dramatically increase sensitivity to LPS through IFN-γ production by CD25⁺Foxp3^- T cells
eLife, Dec 21, 2021; DOI: 10.7554/eLife.62432

Multiparametric flow cytometry and CATALYST analysis

Authors: María M. Coronel, Haval Shirwan and Andrés J. García
Date: Dec 14, 2023，Views：2136

Original protocol used in research article：

Immunotherapy via PD-L1–presenting biomaterials leads to long-term islet graft survival
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.aba5573

PAS, AB, and AB-PAS staining

Authors: Andong Zhao and Xiaobing Fu
Date: Dec 13, 2023，Views：35

Original protocol used in research article：

Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy
Science Advances, Apr 14, 2021; DOI: 10.1126/sciadv.abb2213

Determination of membrane charge

Author: Andrew M Edwards
Date: Dec 12, 2023，Views：56

Original protocol used in research article：

Colistin kills bacteria by targeting lipopolysaccharide in the cytoplasmic membrane
eLife, Apr 6, 2021; DOI: 10.7554/eLife.65836

Immunofluorescent labelling of adult mouse neuromuscular junctions

Authors: Roberta Sartori, Adam Hagg, Paul Gregorevic and Marco Sandri
Date: Dec 7, 2023，Views：2268

Original protocol used in research article：

Perturbed BMP signaling and denervation promote muscle wasting in cancer cachexia
Science Translational Medicine, Aug 4, 2021; DOI: 10.1126/scitranslmed.aay9592

Mouse Aortic Ring Assay

Authors: Carmen Ruiz de Almodovar and Xiaohong Wang
Date: Dec 5, 2023，Views：2520

Original protocol used in research article：

Protein Phosphatase 2A Mediates YAP Activation in Endothelial Cells Upon VEGF Stimulation and Matrix Stiffness
Frontiers in Cell and Developmental Biology, May 13, 2021; DOI: 10.3389/fcell.2021.675562

Myelin phagocytosis and clearance assays

Author: Rasmus Berglund
Date: Dec 4, 2023，Views：2534

Original protocol used in research article：

Microglial autophagy–associated phagocytosis is essential for recovery from neuroinflammation
Science Immunology, Oct 15, 2020; DOI: 10.1126/sciimmunol.abb5077

TeSLA for TL measurement

Author: Jerry W. Shay
Date: Dec 4, 2023，Views：2284

Original protocol used in research article：

A method for measuring the distribution of the shortest telomeres in cells and tissues
Nature Communications, Nov 7, 2017; DOI: 10.1038/s41467-017-01291-z

CRISPR/Cas9-mediated gene editing

Authors: Alyssa N. Werner and Pascale G. Charest
Date: Nov 29, 2023，Views：2552

Original protocol used in research article：

CRISPR-mediated reversion of oncogenic KRAS mutation results in increased proliferation and reveals independent roles of Ras and mTORC2 in the migration of A549 lung cancer cells
Molecular Biology of the Cell, Sep 20, 2023; DOI: 10.1091/mbc.E23-05-0152

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