Protocols shared by authors via RaP service
Journal
Quantification of NaV1.1 Protein in Mouse Brains using Meso Scale Discovery (MSD) Method
Authors: Zhou Han and Gene Liau
Date: Sep 14, 2020，Views：1048
Original protocol used in research article：
Antisense oligonucleotides increase Scn1a expression and reduce seizures and SUDEP incidence in a mouse model of Dravet syndrome
Science Translational Medicine, Aug 26, 2020; DOI: 10.1126/scitranslmed.aaz6100
NK stimulation assay
Author: Sytse Jan Piersma
Date: Sep 14, 2020，Views：945
Original protocol used in research article：
A herpesvirus encoded Qa-1 mimic inhibits natural killer cell cytotoxicity through CD94/NKG2A receptor engagement
eLife, Dec 21, 2018; DOI: 10.7554/eLife.38667
Expression and Purification of the Human Cation-Chloride Cotransporter KCC1 from HEK293F cells for Structural Studies
Authors: Si Liu and Jiangtao Guo
Date: Sep 14, 2020，Views：1219
Original protocol used in research article：
Cryo-EM structures of the human cation-chloride cotransporter KCC1
Science, Oct 25, 2019; DOI: 10.1126/science.aay3129
Human monocyte-derived dendritic cell (moDC) differentiation and suppression of moDCs by Tregs
Authors: Qing Jessica Huang and Megan K. Levings
Date: Sep 13, 2020，Views：1085
Original protocol used in research article：
Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells
Science Translational Medicine, Aug 19, 2020; DOI: 10.1126/scitranslmed.aaz3866
IgH recombination
Author: Masaki Miyazaki
Date: Sep 13, 2020，Views：892
Original protocol used in research article：
The transcription factor E2A activates multiple enhancers that drive Rag expression in developing T and B cells
Science Immunology, Sep 3, 2020; DOI: 10.1126/sciimmunol.abb1455
Synthesis and characterization of CpG-PP, CpG-PM, and CpG-P-ss-M
Authors: Huile Gao and Lin Qin
Date: Sep 10, 2020，Views：979
Original protocol used in research article：
A tumor-to-lymph procedure navigated versatile gel system for combinatorial therapy against tumor recurrence and metastasis
Science Advances, Sep 3, 2020; DOI: 10.1126/sciadv.abb3116
Quantitative RT-PCR analysis
Author: Katherine A Donovan
Date: Sep 10, 2020，Views：1013
Original protocol used in research article：
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
eLife, Aug 1, 2018; DOI: 10.7554/eLife.38430
Detection and quantitation of NAD+- and NADH-capped RNA in vivo: hybridization with a radiolabeled oligodeoxyribonucleotide probe
Author: Jeremy G Bird
Date: Sep 10, 2020，Views：978
Original protocol used in research article：
Highly efficient 5' capping of mitochondrial RNA with NAD+ and NADH by yeast and human mitochondrial RNA polymerase
eLife, Dec 12, 2018; DOI: 10.7554/eLife.42179
Histology and molecular phenotyping
Author: Magda Spella
Date: Sep 9, 2020，Views：978
Original protocol used in research article：
Club cells form lung adenocarcinomas and maintain the alveoli of adult mice
eLife, May 29, 2019; DOI: 10.7554/eLife.45571
Sample preparation - Elemental mapping
Author: Gawain McColl
Date: Sep 9, 2020，Views：931
Original protocol used in research article：
Changes in ferrous iron and glutathione promote ferroptosis and frailty in aging Caenorhabditis elegans
eLife, Jul 21, 2020; DOI: 10.7554/eLife.56580
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.