Bio-101 - Improve Research Reproducibility

HSPC and MPP2 isolation, barcoding, EPO treatment, and transplantation

Authors: Leïla Perié, Jason Cosgrove and Almut Eisele
Date: Feb 7, 2024，Views：931

Original protocol used in research article：

Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells
eLife, Feb 15, 2022; DOI: 10.7554/eLife.66922

BSH Activity Assays.

Authors: Rodolphe Barrangou and Casey M. Theriot
Date: Feb 1, 2024，Views：1021

Original protocol used in research article：

Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization
Proceedings of the National Academy of Sciences of the United States of America, Feb 1, 2021; DOI: 10.1073/pnas.2017709118

Transgenic tomato plants

Authors: A. Stintzi and A. Schaller
Date: Jan 30, 2024，Views：1063

Original protocol used in research article：

Peptide signaling for drought-induced tomato flower drop
Science, Mar 26, 2020; DOI: 10.1126/science.aaz5641

Isolation of human DRG nuclei

Authors: Minh Q Nguyen, Nicholas JP Ryba and Steve Davidson
Date: Jan 30, 2024，Views：1038

Original protocol used in research article：

Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752

PCL nerve conduit fabrication

Authors: Neil B. Fadia and Kacey G. Marra
Date: Jan 26, 2024，Views：1118

Original protocol used in research article：

Long-gap peripheral nerve repair through sustained release of a neurotrophic factor in nonhuman primates
Science Translational Medicine, Jan 21, 2020; DOI: 10.1126/scitranslmed.aav7753

VEGF-A–targeting functional manipulations

Authors: Ralf Paus and Amos Gilhar
Date: Jan 20, 2024，Views：1191

Original protocol used in research article：

Human organ rejuvenation by VEGF-A: Lessons from the skin
Science Advances, Jun 24, 2022; DOI: 10.1126/sciadv.abm6756

8× expansion protocol for the Drosophila CNS

Authors: Joshua L Lillvis, Amy Hu, Paul W Tillberg and Barry J Dickson
Date: Jan 18, 2024，Views：1274

Original protocol used in research article：

Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy
eLife, Oct 26, 2022; DOI: 10.7554/eLife.81248

Immunofluorescence Protocol for HNF4α (P1 and P2 isoforms)

Authors: Karthikeyani Chellappa, Poonamjot Deol and Frances M Sladek
Date: Jan 12, 2024，Views：1440

Original protocol used in research article：

Opposing roles of nuclear receptor HNF4α isoforms in colitis and colitis-associated colon cancer
eLife, May 11, 2016; DOI: 10.7554/eLife.10903

Estimation of the free energy of the Arg381-Asp410 salt bridge

Authors: Yingjie Wang, Gianluigi Veglia, Dongping Zhong and Jiali Gao
Date: Jan 11, 2024，Views：21

Original protocol used in research article：

Activation mechanism of Drosophila cryptochrome through an allosteric switch
Science Advances, Jun 17, 2021; DOI: 10.1126/sciadv.abg3815

In situ hybridizations

Author: Shelby A Blythe
Date: Jan 9, 2024，Views：1567

Original protocol used in research article：

Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network
eLife, Apr 29, 2020; DOI: 10.7554/eLife.53916

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