Bio-101 - Improve Research Reproducibility

TI and strain calculations

Authors: Gustavo R. Perez-Lemus, Juan J. de Pablo, Fabian Bylehn, Cintia Menendez and Walter Alvarado
Date: Mar 11, 2024，Views：885

Original protocol used in research article：

Toward wide-spectrum antivirals against coronaviruses: Molecular characterization of SARS-CoV-2 NSP13 helicase inhibitors
Science Advances, Jan 7, 2022; DOI: 10.1126/sciadv.abj4526

Encapsulation Efficiency (EE) and Loading Efficiency (LE) Protocol

Author: Jiyun Shi
Date: Mar 11, 2024，Views：729

Original protocol used in research article：

A Dual pH-Responsive DOX-Encapsulated Liposome Combined with Glucose Administration Enhanced Therapeutic Efficacy of Chemotherapy for Cancer
International Journal of Nanomedicine, May 10, 2021; DOI: 10.2147/IJN.S303874

Synthesis of FCS

Authors: Xiaofeng Zhang, Ping Gu, Qian Chen and Zhuang Liu
Date: Mar 2, 2024，Views：730

Original protocol used in research article：

Eyedrop-based macromolecular ophthalmic drug delivery for ocular fundus disease treatment
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.abq3104

Liquid food consumption

Author: Patrick T McGrath
Date: Mar 1, 2024，Views：698

Original protocol used in research article：

Changes to social feeding behaviors are not sufficient for fitness gains of the Caenorhabditis elegans N2 reference strain
eLife, Oct 17, 2018; DOI: 10.7554/eLife.38675

Half-Life/Cycloheximide Chase Assay

Authors: Ritu Mishra and Akhil C. Banerjea
Date: Feb 29, 2024，Views：629

Original protocol used in research article：

SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia
Frontiers in Immunology, Apr 14, 2021; DOI: 10.3389/fimmu.2021.656700

Protein sample preparation from Drosophila embryos for Western blot analysis

Author: Ufuk Günesdogan
Date: Feb 16, 2024，Views：1466

Original protocol used in research article：

Histone supply regulates S phase timing and cell cycle progression
eLife, Sep 9, 2014; DOI: 10.7554/eLife.02443

Lentivirus production and transduction

Authors: Wahyu Handoko Wibowo Darsono, Seung-Jae Lee and Sang-Hun Lee
Date: Feb 16, 2024，Views：1182

Original protocol used in research article：

Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson’s disease-linked DNAJC6 mutations
Science Advances, Feb 16, 2021; DOI: 10.1126/sciadv.abb1540

Internalization assay

Authors: Elena Gammella, Alexia Conte, Gaetano Cairo and Stefania Recalcati
Date: Feb 16, 2024，Views：1023

Original protocol used in research article：

Unconventional endocytosis and trafficking of transferrin receptor induced by iron
Molecular Biology of the Cell, Jan 15, 2021; DOI: 10.1091/mbc.E20-02-0129

Dual luciferase assay

Authors: Maija Pollari and Kristiina Mäkinen
Date: Feb 9, 2024，Views：924

Original protocol used in research article：

The potyviral silencing suppressor HCPro recruits and employs host ARGONAUTE1 in pro-viral functions
PLoS Pathogens, Oct 8, 2020; DOI: 10.1371/journal.ppat.1008965

Myelin isolation and staining

Authors: Rasmus Berglund and Maja Jagodic
Date: Feb 8, 2024，Views：1017

Original protocol used in research article：

Microglial autophagy–associated phagocytosis is essential for recovery from neuroinflammation
Science Immunology, Oct 15, 2020; DOI: 10.1126/sciimmunol.abb5077

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