Protocols shared by authors via RaP service
Journal
eIF2α phosphorylation
Authors: Yize Li, Robert H Silverman and Susan R Weiss
Date: Nov 17, 2020，Views：971
Original protocol used in research article：
Ribonuclease L mediates the cell-lethal phenotype of double-stranded RNA editing enzyme ADAR1 deficiency in a human cell line
eLife, Mar 31, 2017; DOI: 10.7554/eLife.25687
Generation of VEGF gradients using vascular bioink
Author: Fiona Freeman
Date: Nov 17, 2020，Views：957
Original protocol used in research article：
3D bioprinting spatiotemporally defined patterns of growth factors to tightly control tissue regeneration
Science Advances, Aug 14, 2020; DOI: 10.1126/sciadv.abb5093
Lactic acid treatment in T cell culture
Author: Franziska M. Uhl
Date: Nov 16, 2020，Views：891
Original protocol used in research article：
Metabolic reprogramming of donor T cells enhances graft-versus-leukemia effects in mice and humans
Science Translational Medicine, Oct 27, 2020; DOI: 10.1126/scitranslmed.abb8969
Dissociation of Epididymis and Vas Deferens
Author: Vera Da Silva Garcia Rinaldi
Date: Nov 15, 2020，Views：938
Original protocol used in research article：
An atlas of cell types in the mouse epididymis and vas deferens
eLife, Jul 30, 2020; DOI: 10.7554/eLife.55474
Subcellular fractionation
Author: Chenglai Fu
Date: Nov 15, 2020，Views：904
Original protocol used in research article：
The inositol pyrophosphate 5-InsP7 drives sodium-potassium pump degradation by relieving an autoinhibitory domain of PI3K p85α
Science Advances, Oct 28, 2020; DOI: 10.1126/sciadv.abb8542
Immunofluorescence
Authors: Kynan T Lawlor and Alexander N Combes
Date: Nov 15, 2020，Views：1461
Original protocol used in research article：
Nephron progenitor commitment is a stochastic process influenced by cell migration
eLife, Jan 24, 2019; DOI: 10.7554/eLife.41156
In vivo fluorescence imaging
Author: Chen Zhou
Date: Nov 15, 2020，Views：841
Original protocol used in research article：
Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis
Science Advances, Sep 10, 2020; DOI: 10.1126/sciadv.abc1586
Isolation of mitochondria
Authors: Nickie C Chan, Grigor Varuzhanyan and David C Chan
Date: Nov 13, 2020，Views：1045
Original protocol used in research article：
Mitochondrial fusion is required for spermatogonial differentiation and meiosis
eLife, Oct 9, 2019; DOI: 10.7554/eLife.51601
Olfactory receptor neuron (ORN) axotomy assay
Author: Maria D Purice
Date: Nov 13, 2020，Views：1105
Original protocol used in research article：
A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade
eLife, Aug 21, 2017; DOI: 10.7554/eLife.23611
T-maze, delayed non-match to place version
Authors: Da Hee Jung and Byung Tae Choi
Date: Nov 12, 2020，Views：1350
Original protocol used in research article：
Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56359
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