Protocols shared by authors via RaP service
Journal
Phenotyping of adoptively transferred and endogenous tumor-infiltrating CD8+ T cells in the B16F10 orthotopic melanoma model
Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Dec 8, 2020，Views：901
Original protocol used in research article：
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667
Flow cytometry dissociation protocol for Individual Drosophila brains
Author: Shyama Nandakumar
Date: Dec 7, 2020，Views：1080
Original protocol used in research article：
Polyploidy in the adult Drosophila brain
eLife, Aug 25, 2020; DOI: 10.7554/eLife.54385
Evaluating T cell escape in epitopes previously defined as reactive in clade B consensus mapping - Protocol for Elispot, Peptide handling
Authors: Joanna A Warren and Nilu Goonetilleke
Date: Dec 7, 2020，Views：1029
Original protocol used in research article：
The HIV-1 latent reservoir is largely sensitive to circulating T cells
eLife, Oct 6, 2020; DOI: 10.7554/eLife.57246
Transfection, pool maintenance, and drug addition
Authors: Raghuvir Viswanatha and Norbert Perrimon
Date: Dec 7, 2020，Views：1009
Original protocol used in research article：
Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells
eLife, Jul 27, 2018; DOI: 10.7554/eLife.36333
Stimuli
Author: Paolo Ossola
Date: Dec 7, 2020，Views：930
Original protocol used in research article：
Belief updating in bipolar disorder predicts time of recurrence
eLife, Nov 10, 2020; DOI: 10.7554/eLife.58891
Single-cell RNA-seq analysis of public 10x data
Authors: Jean-Baptiste Gorin, Vincent Wu, Son Nguyen and Marcus Buggert
Date: Dec 7, 2020，Views：850
Original protocol used in research article：
TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918
Isolation of single cells from cryoinjured hearts
Author: Jeroen Bakkers
Date: Dec 5, 2020，Views：928
Original protocol used in research article：
Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart
eLife, Dec 23, 2019; DOI: 10.7554/eLife.50163
Demographic history with dadi
Author: Ira Cooke
Date: Dec 4, 2020，Views：883
Original protocol used in research article：
Genomic signatures in the coral holobiont reveal host adaptations driven by Holocene climate change and reef specific symbionts
Science Advances, Nov 26, 2020; DOI: 10.1126/sciadv.abc6318
Human specimens, neuropathological evaluation, and criteria of diagnosis
Author: Eliezer Masliah
Date: Dec 4, 2020，Views：876
Original protocol used in research article：
LRRK2 mediates microglial neurotoxicity via NFATc2 in rodent models of synucleinopathies
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.aay0399
ChIP-seq
Author: Randall H Morse
Date: Dec 4, 2020，Views：940
Original protocol used in research article：
Role of the pre-initiation complex in Mediator recruitment and dynamics
eLife, Dec 12, 2018; DOI: 10.7554/eLife.39633
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