Bio-101 - Improve Research Reproducibility

TRACE-seq Data analysis

Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Nov 10, 2020，Views：1049

Original protocol used in research article：

Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919

Preparation of monobody mRNA libraries for selection against SARS-CoV-2 targets

Authors: Taishi Kondo and Hiroshi Murakami
Date: Nov 9, 2020，Views：1154

Original protocol used in research article：

Antibody-like proteins that capture and neutralize SARS-CoV-2
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3916

Protocols for FACS purification of cardiac total interstitial single cells from adult mouse cardiac ventricles

Authors: Nona Farbehi, Vaibhao Janbandhu, Ralph Patrick, Munira Xaymardan, Robert R Nordon and Richard P Harvey
Date: Nov 9, 2020，Views：1219

Original protocol used in research article：

Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882

rRNA depletion RNAseq protocol

Author: Ye Duan
Date: Nov 9, 2020，Views：1040

Original protocol used in research article：

RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans
microPublication Biology, Sep 22, 2020; DOI: 10.17912/micropub.biology.000312

Isolation, culture and identification of primary Müller cells from the macula and peripheral human retina

Authors: Ting Zhang, Yingying Chen, Shaoxue Zeng and Ling Zhu
Date: Nov 8, 2020，Views：1218

Original protocol used in research article：

Human macular Müller cells rely more on serine biosynthesis to combat oxidative stress than those from the periphery
eLife, Apr 30, 2019; DOI: 10.7554/eLife.43598

Optogenetic stimulation

Author: Shi-Bin Li
Date: Nov 7, 2020，Views：956

Original protocol used in research article：

Hypothalamic circuitry underlying stress-induced insomnia and peripheral immunosuppression
Science Advances, Sep 9, 2020; DOI: 10.1126/sciadv.abc2590

Chromatin immunoprecipitation (ChIP) protocol for the sponge Amphimedon queenslandica

Authors: Federico Gaiti, Bernard M Degnan and Miloš Tanurdžić
Date: Nov 7, 2020，Views：878

Original protocol used in research article：

Landscape of histone modifications in a sponge reveals the origin of animal cis-regulatory complexity
eLife, Apr 11, 2017; DOI: 10.7554/eLife.22194

GeneWeld: a method for efficient targeted integration directed by short homology

Authors: Maura McGrail and Jeffrey Essner
Date: Nov 7, 2020，Views：1550

Original protocol used in research article：

Efficient targeted integration directed by short homology in zebrafish and mammalian cells
eLife, May 15, 2020; DOI: 10.7554/eLife.53968

Splicing efficiency and poly(A) tail length/site

Authors: Daniel R Larson and Murali Palangat
Date: Nov 7, 2020，Views：932

Original protocol used in research article：

Kinetic competition during the transcription cycle results in stochastic RNA processing
eLife, Oct 1, 2014; DOI: 10.7554/eLife.03939

T1RESS sequence design and scan parameters

Author: Robert R Edelman
Date: Nov 6, 2020，Views：856

Original protocol used in research article：

Twofold improved tumor-to-brain contrast using a novel T1 relaxation-enhanced steady-state (T1RESS) MRI technique
Science Advances, Oct 28, 2020; DOI: 10.1126/sciadv.abd1635

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