Bio-101 - Improve Research Reproducibility

Isolation, culture and identification of primary Müller cells from the macula and peripheral human retina

Authors: Ting Zhang, Yingying Chen, Shaoxue Zeng and Ling Zhu
Date: Nov 8, 2020，Views：1234

Original protocol used in research article：

Human macular Müller cells rely more on serine biosynthesis to combat oxidative stress than those from the periphery
eLife, Apr 30, 2019; DOI: 10.7554/eLife.43598

Optogenetic stimulation

Author: Shi-Bin Li
Date: Nov 7, 2020，Views：966

Original protocol used in research article：

Hypothalamic circuitry underlying stress-induced insomnia and peripheral immunosuppression
Science Advances, Sep 9, 2020; DOI: 10.1126/sciadv.abc2590

Chromatin immunoprecipitation (ChIP) protocol for the sponge Amphimedon queenslandica

Authors: Federico Gaiti, Bernard M Degnan and Miloš Tanurdžić
Date: Nov 7, 2020，Views：897

Original protocol used in research article：

Landscape of histone modifications in a sponge reveals the origin of animal cis-regulatory complexity
eLife, Apr 11, 2017; DOI: 10.7554/eLife.22194

GeneWeld: a method for efficient targeted integration directed by short homology

Authors: Maura McGrail and Jeffrey Essner
Date: Nov 7, 2020，Views：1568

Original protocol used in research article：

Efficient targeted integration directed by short homology in zebrafish and mammalian cells
eLife, May 15, 2020; DOI: 10.7554/eLife.53968

Splicing efficiency and poly(A) tail length/site

Authors: Daniel R Larson and Murali Palangat
Date: Nov 7, 2020，Views：956

Original protocol used in research article：

Kinetic competition during the transcription cycle results in stochastic RNA processing
eLife, Oct 1, 2014; DOI: 10.7554/eLife.03939

T1RESS sequence design and scan parameters

Author: Robert R Edelman
Date: Nov 6, 2020，Views：869

Original protocol used in research article：

Twofold improved tumor-to-brain contrast using a novel T1 relaxation-enhanced steady-state (T1RESS) MRI technique
Science Advances, Oct 28, 2020; DOI: 10.1126/sciadv.abd1635

Nanogel preparation and characterization

Author: Xiao Xu
Date: Nov 6, 2020，Views：949

Original protocol used in research article：

Self-regulated hirudin delivery for anticoagulant therapy
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abc0382

Crispr-CAS9 knockout of Piezo1

Author: Josefina Ines del Marmol
Date: Nov 5, 2020，Views：989

Original protocol used in research article：

Piezo1 forms a slowly-inactivating mechanosensory channel in mouse embryonic stem cells
eLife, Aug 22, 2018; DOI: 10.7554/eLife.33149

Assay protocols

Authors: Ali H. Alghamdi and Harry P. De Koning
Date: Nov 5, 2020，Views：1142

Original protocol used in research article：

Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416

Construction of the BiG Mito-Split-GFP strain

Authors: Roza Kucharczyk and Hubert Dominique Becker
Date: Nov 5, 2020，Views：1162

Original protocol used in research article：

Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP
eLife, Jul 13, 2020; DOI: 10.7554/eLife.56649

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