Protocols shared by authors via RaP service
Journal
Nanogel preparation and characterization
Author: Xiao Xu
Date: Nov 6, 2020，Views：938
Original protocol used in research article：
Self-regulated hirudin delivery for anticoagulant therapy
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abc0382
Crispr-CAS9 knockout of Piezo1
Author: Josefina Ines del Marmol
Date: Nov 5, 2020，Views：962
Original protocol used in research article：
Piezo1 forms a slowly-inactivating mechanosensory channel in mouse embryonic stem cells
eLife, Aug 22, 2018; DOI: 10.7554/eLife.33149
Assay protocols
Authors: Ali H. Alghamdi and Harry P. De Koning
Date: Nov 5, 2020，Views：1117
Original protocol used in research article：
Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416
Construction of the BiG Mito-Split-GFP strain
Authors: Roza Kucharczyk and Hubert Dominique Becker
Date: Nov 5, 2020，Views：1137
Original protocol used in research article：
Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP
eLife, Jul 13, 2020; DOI: 10.7554/eLife.56649
Single cell library preparation
Author: Christopher A Jackson
Date: Nov 5, 2020，Views：1014
Original protocol used in research article：
Gene regulatory network reconstruction using single-cell RNA sequencing of barcoded genotypes in diverse environments
eLife, Jan 27, 2020; DOI: 10.7554/eLife.51254
Molecular Dynamics simulations
Authors: Alzbeta Tuerkova, Robert Vácha and Ivo Kabelka
Date: Nov 4, 2020，Views：2518
Original protocol used in research article：
Effect of helical kink in antimicrobial peptides on membrane pore formation
eLife, Mar 13, 2020; DOI: 10.7554/eLife.47946
Single-cell RNA sequencing of endosteal bone marrow cells
Author: Leilei Zhong
Date: Nov 3, 2020，Views：1134
Original protocol used in research article：
Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment
eLife, Apr 14, 2020; DOI: 10.7554/eLife.54695
CRISPR-Cas9 sgRNA design and construction
Author: Yen-Lin Huang
Date: Nov 3, 2020，Views：2086
Original protocol used in research article：
Collagen-rich omentum is a premetastatic niche for integrin α2-mediated peritoneal metastasis
eLife, Oct 7, 2020; DOI: 10.7554/eLife.59442
Hfq UV cross-linking, ligation and analysis of hybrids (Hfq-CLASH)
Authors: Stuart William Mckellar, Ira Alexandra Iosub, Ivayla Ivanova and Sander Granneman
Date: Nov 3, 2020，Views：950
Original protocol used in research article：
Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation
eLife, May 1, 2020; DOI: 10.7554/eLife.54655
Generation of Gadl1-null mice
Author: Elaheh Mahootchi
Date: Nov 3, 2020，Views：839
Original protocol used in research article：
GADL1 is a multifunctional decarboxylase with tissue-specific roles in β-alanine and carnosine production
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.abb3713
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.