Protocols shared by authors via RaP service
Journal
Isolation of single cells from cryoinjured hearts
Author: Jeroen Bakkers
Date: Dec 5, 2020，Views：929
Original protocol used in research article：
Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart
eLife, Dec 23, 2019; DOI: 10.7554/eLife.50163
Demographic history with dadi
Author: Ira Cooke
Date: Dec 4, 2020，Views：884
Original protocol used in research article：
Genomic signatures in the coral holobiont reveal host adaptations driven by Holocene climate change and reef specific symbionts
Science Advances, Nov 26, 2020; DOI: 10.1126/sciadv.abc6318
Human specimens, neuropathological evaluation, and criteria of diagnosis
Author: Eliezer Masliah
Date: Dec 4, 2020，Views：876
Original protocol used in research article：
LRRK2 mediates microglial neurotoxicity via NFATc2 in rodent models of synucleinopathies
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.aay0399
ChIP-seq
Author: Randall H Morse
Date: Dec 4, 2020，Views：942
Original protocol used in research article：
Role of the pre-initiation complex in Mediator recruitment and dynamics
eLife, Dec 12, 2018; DOI: 10.7554/eLife.39633
Primers for Quantitative real-time PCR
Authors: Shunxing Rong and Jay D Horton
Date: Dec 4, 2020，Views：1152
Original protocol used in research article：
Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice
eLife, Feb 28, 2017; DOI: 10.7554/eLife.25015
Programming and reading schemes of the memristor-based system
Author: Jianshi Tang
Date: Dec 4, 2020，Views：892
Original protocol used in research article：
Multichannel parallel processing of neural signals in memristor arrays
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abc4797
Acid-base titration
Author: Funeng Xu
Date: Dec 4, 2020，Views：896
Original protocol used in research article：
Development of a pH-responsive polymersome inducing endoplasmic reticulum stress and autophagy blockade
Science Advances, Jul 30, 2020; DOI: 10.1126/sciadv.abb8725
Staff screening protocols
Author: Nick K Jones
Date: Dec 3, 2020，Views：1000
Original protocol used in research article：
Effective control of SARS-CoV-2 transmission between healthcare workers during a period of diminished community prevalence of COVID-19
eLife, Jun 19, 2020; DOI: 10.7554/eLife.59391
Sphere culture
Author: Tanya Das
Date: Dec 3, 2020，Views：883
Original protocol used in research article：
SMAR1 repression by pluripotency factors and consequent chemoresistance in breast cancer stem-like cells is reversed by aspirin
Science Signaling, Oct 19, 2020; DOI: 10.1126/scisignal.aay6077
CRISPR/Cas9-mediated editing and cell sorting
Authors: Li Fan and Honglin Wang
Date: Dec 2, 2020，Views：948
Original protocol used in research article：
A micropeptide encoded by lncRNA MIR155HG suppresses autoimmune inflammation via modulating antigen presentation
Science Advances, May 20, 2020; DOI: 10.1126/sciadv.aaz2059
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