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Recombinant expression and purification of human EP300-KIX87 domain from Escherichia coli
Authors: John H. Bushweller, Kwon-Sik Park and Ashish Kabra
Date: Mar 21, 2024，Views：869
Original protocol used in research article：
KIX domain determines a selective tumor-promoting role for EP300 and its vulnerability in small cell lung cancer
Science Advances, Feb 16, 2022; DOI: 10.1126/sciadv.abl4618
Construct design
Authors: Arpita Bharadwaj and Isabelle Baconguis
Date: Mar 20, 2024，Views：874
Original protocol used in research article：
Structure of the human epithelial sodium channel by cryo-electron microscopy
eLife, Sep 25, 2018; DOI: 10.7554/eLife.39340
Reporter design and virus production
Authors: Natalya N Pavlova and Craig B Thompson
Date: Mar 20, 2024，Views：860
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
Protein constructs, expression, and purification
Authors: Alexei Gorelik and Bhushan Nagar
Date: Mar 19, 2024，Views：874
Original protocol used in research article：
Structure of the immunoregulatory sialidase NEU1
Science Advances, May 19, 2023; DOI: 10.1126/sciadv.adf8169
UMAP and ICI analysis
Authors: Bruno Guillotin and Kenneth D. Birnbaum
Date: Mar 19, 2024，Views：42
Original protocol used in research article：
Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327
Community network analysis
Authors: Francesco Colizzi and Modesto Orozco
Date: Mar 18, 2024，Views：29
Original protocol used in research article：
Probing allosteric regulations with coevolution-driven molecular simulations
Science Advances, Sep 8, 2021; DOI: 10.1126/sciadv.abj0786
Small RNA preparation and qPCR
Authors: Luxi Chen and Anming Meng
Date: Mar 18, 2024，Views：813
Original protocol used in research article：
5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
Science Advances, Nov 19, 2021; DOI: 10.1126/sciadv.abh0494
Purification of S. aureus ClpC and ClpP
Authors: Marta Carroni, Bernd Bukau and Axel Mogk
Date: Mar 17, 2024，Views：870
Original protocol used in research article：
Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control
eLife, Nov 22, 2017; DOI: 10.7554/eLife.30120
Ferrozine Iron Assay
Author: Kuanyu Li
Date: Mar 12, 2024，Views：947
Original protocol used in research article：
Hormone replacement therapy for postmenopausal atherosclerosis is offset by late age iron deposition
eLife, Aug 10, 2023; DOI: 10.7554/eLife.80494
PCA and DCCM
Authors: Gustavo R. Perez-Lemus, Juan J. de Pablo, Fabian Bylehn, Cintia Menendez and Walter Alvarado
Date: Mar 11, 2024，Views：854
Original protocol used in research article：
Toward wide-spectrum antivirals against coronaviruses: Molecular characterization of SARS-CoV-2 NSP13 helicase inhibitors
Science Advances, Jan 7, 2022; DOI: 10.1126/sciadv.abj4526
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