Protocols shared by authors via RaP service
Journal
TGIRT-seq library construction
Author: Alan M Lambowitz
Date: Dec 22, 2020，Views：1180
Original protocol used in research article：
Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling
eLife, Sep 2, 2020; DOI: 10.7554/eLife.60743
ATAC sequencing protocol starting from frozen cells
Authors: Monika Mehta and Juan Manuel Caravaca-Guasch
Date: Dec 22, 2020，Views：957
Original protocol used in research article：
Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847
in vitro Fluorescence Resonance Energy Transfer (FRET) and FRET in cells
Authors: Seunghan Son and Chul Geun Kim
Date: Dec 22, 2020，Views：821
Original protocol used in research article：
Rational discovery of antimetastatic agents targeting the intrinsically disordered region of MBD2
Science Advances, Nov 19, 2019; DOI: 10.1126/sciadv.aav9810
Sequencing data processing and analysis
Authors: Alexandra Popa, Jakob-Wendelin Genger and Andreas Bergthaler
Date: Dec 22, 2020，Views：997
Original protocol used in research article：
Genomic epidemiology of superspreading events in Austria reveals mutational dynamics and transmission properties of SARS-CoV-2
Science Translational Medicine, Dec 9, 2020; DOI: 10.1126/scitranslmed.abe2555
Tracking subcellular localization of surface proteins in Staphylococcus aureus by immunofluorescence microscopy
Authors: Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu
Date: Dec 21, 2020，Views：1183
Original protocol used in research article：
Septal secretion of protein A in Staphylococcus aureus requires SecA and lipoteichoic acid synthesis
eLife, May 14, 2018; DOI: 10.7554/eLife.34092
Cell culture and adipocyte differentiation
Authors: Marcelo A Mori and Andréa Livia Rocha
Date: Dec 21, 2020，Views：1739
Original protocol used in research article：
Enoxacin induces oxidative metabolism and mitigates obesity by regulating adipose tissue miRNA expression
Science Advances, Dec 2, 2020; DOI: 10.1126/sciadv.abc6250
Animals and treatments
Author: Hui Xie
Date: Dec 20, 2020，Views：928
Original protocol used in research article：
Ångstrom-scale silver particle–embedded carbomer gel promotes wound healing by inhibiting bacterial colonization and inflammation
Science Advances, Oct 23, 2020; DOI: 10.1126/sciadv.aba0942
Urea avidity ELISA
Author: Harry N White
Date: Dec 19, 2020，Views：1164
Original protocol used in research article：
Variant proteins stimulate more IgM+ GC B-cells revealing a mechanism of cross-reactive recognition by antibody memory
eLife, May 1, 2018; DOI: 10.7554/eLife.26832
Ubiquitylation assays
Author: Joseph S Harrison
Date: Dec 18, 2020，Views：895
Original protocol used in research article：
Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1
eLife, Sep 6, 2016; DOI: 10.7554/eLife.17101
RNA library preparation
Author: Ryan Muller
Date: Dec 16, 2020，Views：986
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
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