Protocols shared by authors via RaP service
Journal
Flow cytometry analysis
Author: Hong-Ru Chen
Date: Jan 19, 2021，Views：835
Original protocol used in research article：
Fate mapping via CCR2-CreER mice reveals monocyte-to-microglia transition in development and neonatal stroke
Science Advances, Aug 25, 2020; DOI: 10.1126/sciadv.abb2119
Immunohistochemistry
Author: Hong-Ru Chen
Date: Jan 19, 2021，Views：849
Original protocol used in research article：
Fate mapping via CCR2-CreER mice reveals monocyte-to-microglia transition in development and neonatal stroke
Science Advances, Aug 25, 2020; DOI: 10.1126/sciadv.abb2119
Traumatic brain injury
Authors: Rebekah Mannix, Wen Li and Jianhua Qiu
Date: Jan 19, 2021，Views：1048
Original protocol used in research article：
BBB pathophysiology–independent delivery of siRNA in traumatic brain injury
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd6889
CRISPR/Cas9-mediated targeting of TINF2 in RPE-1 cells
Author: Isabelle Schmutz
Date: Jan 19, 2021，Views：1212
Original protocol used in research article：
TINF2 is a haploinsufficient tumor suppressor that limits telomere length
eLife, Dec 1, 2020; DOI: 10.7554/eLife.61235
Calculation of the divergence rate
Author: Germán Plata
Date: Jan 18, 2021，Views：949
Original protocol used in research article：
Molecular function limits divergent protein evolution on planetary timescales
eLife, Sep 18, 2019; DOI: 10.7554/eLife.39705
Cell lines and in vitro culture conditions
Authors: Qianru Jin, David H. Gracias and Lewis H. Romer
Date: Jan 17, 2021，Views：843
Original protocol used in research article：
Biomimetic human small muscular pulmonary arteries
Science Advances, Mar 24, 2020; DOI: 10.1126/sciadv.aaz2598
Observational fear response (Social Transfer of Fear)
Author: Monique L. Smith
Date: Jan 16, 2021，Views：920
Original protocol used in research article：
Anterior cingulate inputs to nucleus accumbens control the social transfer of pain and analgesia
Science, Jan 8, 2021; DOI: 10.1126/science.abe3040
Cell culture
Authors: Manuel Kaulich and Steven F Dowdy
Date: Jan 15, 2021，Views：1179
Original protocol used in research article：
Cyclin D activates the Rb tumor suppressor by mono-phosphorylation
eLife, Jun 4, 2014; DOI: 10.7554/eLife.02872
Behavioral assays
Authors: Charles A Hoeffer and Helen Wong
Date: Jan 15, 2021，Views：970
Original protocol used in research article：
Isoform-specific roles for AKT in affective behavior, spatial memory, and extinction related to psychiatric disorders
eLife, Dec 16, 2020; DOI: 10.7554/eLife.56630
Assays of tagmentation activity of Tn5 on RNA/DNA hybrids
Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Jan 15, 2021，Views：1124
Original protocol used in research article：
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.