Protocols shared by authors via RaP service
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Tn5 in vitro tagmentation on RNA/DNA hybrids
Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Jan 15, 2021，Views：1305
Original protocol used in research article：
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919
TRACE-seq library preparation and sequencing
Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Jan 15, 2021，Views：1234
Original protocol used in research article：
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919
Diffusion-weighted MRI protocol for acute brain edema detection after middle cerebral artery occlusion in mice
Authors: Humberto Mestre, Ting Du, Maiken Nedergaard and Yuki Mori
Date: Jan 15, 2021，Views：4124
Original protocol used in research article：
Cerebrospinal fluid influx drives acute ischemic tissue swelling
Science, Mar 12, 2020; DOI: 10.1126/science.aax7171
CUT&RUN protocol for egg chambers
Authors: Elizabeth M Knapp, Wei Li and Jianjun Sun
Date: Jan 15, 2021，Views：1087
Original protocol used in research article：
Nuclear receptor Ftz-f1 promotes follicle maturation and ovulation partly via bHLH/PAS transcription factor Sim
eLife, Apr 27, 2020; DOI: 10.7554/eLife.54568
Metagenome annotation with dbCAN and AntiSMASH
Author: Víctor J. Carrión
Date: Jan 15, 2021，Views：935
Original protocol used in research article：
Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome
Science, Nov 1, 2019; DOI: 10.1126/science.aaw9285
Soil collection, plant bioassays and endosphere sampling
Author: Víctor J. Carrión
Date: Jan 15, 2021，Views：1025
Original protocol used in research article：
Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome
Science, Nov 1, 2019; DOI: 10.1126/science.aaw9285
HCR on Whole Mouse Embryos
Authors: Matthew J Anderson and Mark Lewandoski
Date: Jan 13, 2021，Views：1032
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
CRISPR constructs
Author: Kui Li
Date: Jan 12, 2021，Views：928
Original protocol used in research article：
CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance
eLife, Sep 2, 2020; DOI: 10.7554/eLife.57132
Immunofluorescence
Author: Shanshan Gao
Date: Jan 12, 2021，Views：791
Original protocol used in research article：
TIP60 K430 SUMOylation attenuates its interaction with DNA-PKcs in S-phase cells: Facilitating homologous recombination and emerging target for cancer therapy
Science Advances, Jul 9, 2020; DOI: 10.1126/sciadv.aba7822
Study design
Author: Hubert H. Lim
Date: Jan 11, 2021，Views：883
Original protocol used in research article：
Bimodal neuromodulation combining sound and tongue stimulation reduces tinnitus symptoms in a large randomized clinical study
Science Translational Medicine, Oct 6, 2020; DOI: 10.1126/scitranslmed.abb2830
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