Protocols shared by authors via RaP service
Journal
Constructs and stable cell lines
Author: Naeha Subramanian
Date: Dec 28, 2020，Views：976
Original protocol used in research article：
A small sustained increase in NOD1 abundance promotes ligand-independent inflammatory and oncogene transcriptional responses
Science Signaling, Dec 7, 2020; DOI: 10.1126/scisignal.aba3244
PBMC processing and stimulations
Authors: Thanaphong Phongpreecha, Rosemary Fernandez and Thomas Montine
Date: Dec 24, 2020，Views：1061
Original protocol used in research article：
Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575
Lateral hypothalamus surgery
Author: Yeka Aponte
Date: Dec 22, 2020，Views：935
Original protocol used in research article：
High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo
eLife, Jan 4, 2019; DOI: 10.7554/eLife.40805
In vitro cleavage assay and Electrophoretic Mobility Shift Assay (EMSA)
Author: Roger S. Zou
Date: Dec 22, 2020，Views：1081
Original protocol used in research article：
Very fast CRISPR on demand
Science, Jun 11, 2020; DOI: 10.1126/science.aay8204
TGIRT-seq library construction
Author: Alan M Lambowitz
Date: Dec 22, 2020，Views：1179
Original protocol used in research article：
Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling
eLife, Sep 2, 2020; DOI: 10.7554/eLife.60743
ATAC sequencing protocol starting from frozen cells
Authors: Monika Mehta and Juan Manuel Caravaca-Guasch
Date: Dec 22, 2020，Views：952
Original protocol used in research article：
Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847
in vitro Fluorescence Resonance Energy Transfer (FRET) and FRET in cells
Authors: Seunghan Son and Chul Geun Kim
Date: Dec 22, 2020，Views：814
Original protocol used in research article：
Rational discovery of antimetastatic agents targeting the intrinsically disordered region of MBD2
Science Advances, Nov 19, 2019; DOI: 10.1126/sciadv.aav9810
Sequencing data processing and analysis
Authors: Alexandra Popa, Jakob-Wendelin Genger and Andreas Bergthaler
Date: Dec 22, 2020，Views：993
Original protocol used in research article：
Genomic epidemiology of superspreading events in Austria reveals mutational dynamics and transmission properties of SARS-CoV-2
Science Translational Medicine, Dec 9, 2020; DOI: 10.1126/scitranslmed.abe2555
Tracking subcellular localization of surface proteins in Staphylococcus aureus by immunofluorescence microscopy
Authors: Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu
Date: Dec 21, 2020，Views：1177
Original protocol used in research article：
Septal secretion of protein A in Staphylococcus aureus requires SecA and lipoteichoic acid synthesis
eLife, May 14, 2018; DOI: 10.7554/eLife.34092
Cell culture and adipocyte differentiation
Authors: Marcelo A Mori and Andréa Livia Rocha
Date: Dec 21, 2020，Views：1735
Original protocol used in research article：
Enoxacin induces oxidative metabolism and mitigates obesity by regulating adipose tissue miRNA expression
Science Advances, Dec 2, 2020; DOI: 10.1126/sciadv.abc6250
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