Protocols shared by authors via RaP service
Journal
Whole-mount in situ hybridization of Hydra
Authors: Hiroyuki J. Kanaya, Yoshitaka Kobayakawa and Taichi Q. Itoh
Date: Jan 24, 2021，Views：963
Original protocol used in research article：
A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb9415
Differential gene expression analysis of bulk RNA-seq data
Author: Mohamed Nadhir Djekidel
Date: Jan 24, 2021，Views：969
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
DNA library preparation
Authors: Ryan Muller and Nicholas T. Ingolia
Date: Jan 24, 2021，Views：1068
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Plasmid library construction
Authors: Ryan Muller and Nicholas T Ingolia
Date: Jan 24, 2021，Views：1145
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Genomic annotation files preparation
Author: Mohamed Nadhir Djekidel
Date: Jan 24, 2021，Views：1410
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
Extracorporeal circulation treatment
Author: He-Xin Yan
Date: Jan 24, 2021，Views：751
Original protocol used in research article：
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aba5146
Cell culture and hepatocytes optimization
Author: Weijian Li
Date: Jan 24, 2021，Views：796
Original protocol used in research article：
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aba5146
Repeat pseudo-genome preparation
Author: Mohamed Nadhir Djekidel
Date: Jan 23, 2021，Views：1093
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
Immunohistochemistry and imaging
Author: Mehak M Khan
Date: Jan 23, 2021，Views：839
Original protocol used in research article：
Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions
eLife, Apr 29, 2020; DOI: 10.7554/eLife.55165
Expression variability estimation
Author: Benjamin Jung Fair
Date: Jan 22, 2021，Views：975
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
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