Bio-101 - Improve Research Reproducibility

Protocol for preparation of Quantum dot loaded dissolvable microneedle patches

Authors: Ana Jaklenec, Robert Langer and Kevin J. McHugh
Date: Apr 11, 2024，Views：980

Original protocol used in research article：

Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination
Science Translational Medicine, Dec 17, 2019; DOI: 10.1126/scitranslmed.aay7162

Stimulus sets in the study entitled 'Emerged human-like facial expression representation in a deep convolutional neural network'

Authors: Liqin Zhou, Ming Meng, Ke Zhou and Anmin Yang
Date: Apr 10, 2024，Views：28

Original protocol used in research article：

Emerged human-like facial expression representation in a deep convolutional neural network
Science Advances, Mar 23, 2022; DOI: 10.1126/sciadv.abj4383

Quantitative assessment of Stat92E-GFP expression and anti–p-Stat staining during oogenesis

Authors: Jhen-Wei Wu, Yu-Chiuan Chang and Anna C.-C. Jang
Date: Apr 1, 2024，Views：15

Original protocol used in research article：

Spatiotemporal gating of Stat nuclear influx by Drosophila Npas4 in collective cell migration
Science Advances, Jul 22, 2022; DOI: 10.1126/sciadv.abm2411

Isolation of Lamina Propria Cells from Mouse Small and Large Intestines

Authors: Qing Zhao and Charles O. Elson
Date: Mar 29, 2024，Views：765

Original protocol used in research article：

CD4+ T cell activation and concomitant mTOR metabolic inhibition can ablate microbiota-specific memory cells and prevent colitis
Science Immunology, Dec 11, 2020; DOI: 10.1126/sciimmunol.abc6373

Sulfate detection by ion-exchange chromatography

Authors: Maria Dalgaard Mikkelsen and Anne S. Meyer
Date: Mar 29, 2024，Views：27

Original protocol used in research article：

A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure
Scientific Reports, Sep 30, 2021; DOI: 10.1038/s41598-021-98588-3

T cell transwell migration

Authors: Scott B Thompson and Jordan Jacobelli
Date: Mar 27, 2024，Views：1116

Original protocol used in research article：

Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046

Measurement of luciferase activity in tobacco

Author: Ken-ichiro Taoka
Date: Mar 26, 2024，Views：854

Original protocol used in research article：

Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc
Plant Biotechnology, Mar 25, 2021; DOI: 10.5511/plantbiotechnology.20.1209a

Analyses of single-molecule TIRF data

Authors: Deepti Karandur, Evan R Williams and John Kuriyan
Date: Mar 26, 2024，Views：54

Original protocol used in research article：

Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
eLife, Sep 9, 2020; DOI: 10.7554/eLife.57784

Immunostaining protocol of human 3D bioengineered skeletal muscle whole mounts and cross section for confocal microscopy

Authors: Arne D Hofemeier and Timo Betz
Date: Mar 26, 2024，Views：833

Original protocol used in research article：

Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
eLife, Jan 18, 2021; DOI: 10.7554/eLife.60145

Adult rat DRG neuron culture

Authors: Dong Wang, Susu Mao and Bin Yu
Date: Mar 25, 2024，Views：820

Original protocol used in research article：

Promoting axon regeneration by inhibiting RNA N6-methyladenosine demethylase ALKBH5
eLife, Aug 3, 2023; DOI: 10.7554/eLife.85309

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