Protocols shared by authors via RaP service
Journal
Mating assays
Author: Patricia J Wittkopp
Date: Feb 17, 2021，Views：873
Original protocol used in research article：
The yellow gene influences Drosophila male mating success through sex comb melanization
eLife, Oct 15, 2019; DOI: 10.7554/eLife.49388
VivosX assay for MCF10A Cells
Authors: Lisa M Kim and Cheuk T Leung
Date: Feb 17, 2021，Views：874
Original protocol used in research article：
VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells
eLife, Aug 9, 2018; DOI: 10.7554/eLife.36654
MACS sorting of TAMs from human and mouse lung tumor tissues
Authors: Poonam Ashwin Kumar Sarode and Rajkumar Savai
Date: Feb 17, 2021，Views：878
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
mRNAs half-lives determination
Author: Ning Dai
Date: Feb 16, 2021，Views：867
Original protocol used in research article：
IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
eLife, Jul 28, 2017; DOI: 10.7554/eLife.27155
Protein expression, purification, and site-specific labeling
Author: Jesper Levring
Date: Feb 16, 2021，Views：1003
Original protocol used in research article：
Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451
Cloning of mlCNBD-FRET
Author: Dagmar Wachten
Date: Feb 16, 2021，Views：991
Original protocol used in research article：
A novel biosensor to study cAMP dynamics in cilia and flagella
eLife, Mar 22, 2016; DOI: 10.7554/eLife.14052
Muscle myoblast isolation and culture
Author: Shihuan Kuang
Date: Feb 15, 2021，Views：943
Original protocol used in research article：
Stage-specific effects of Notch activation during skeletal myogenesis
eLife, Sep 19, 2016; DOI: 10.7554/eLife.17355
Multiplex FISH in CaRMA-imaged tissue after in vivo calcium imaging
Authors: Hui Yang, Shengjin Xu and Scott M. Sternson
Date: Feb 15, 2021，Views：991
Original protocol used in research article：
Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI: 10.1126/science.abb2494
Alignment of the GRIN lens
Authors: Zhongya Qin, Nancy Y. Ip and Jianan Y. Qu
Date: Feb 15, 2021，Views：1011
Original protocol used in research article：
Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes
Science Advances, Sep 29, 2020; DOI: 10.1126/sciadv.abc6521
HCN4 immunostaining in human (Alomone labs antibody)
Authors: Angélique Bordey and Lena Nguyen
Date: Feb 13, 2021，Views：831
Original protocol used in research article：
Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Science Translational Medicine, Nov 17, 2020; DOI: 10.1126/scitranslmed.abc1492
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