Protocols shared by authors via RaP service
Journal
Cryo-EM data collection
Authors: Sunbin Deng and Ronen Marmorstein
Date: Mar 18, 2021，Views：824
Original protocol used in research article：
Molecular basis for N-terminal alpha-synuclein acetylation by human NatB
eLife, Sep 4, 2020; DOI: 10.7554/eLife.57491
PIV
Authors: James F Pelletier and Timothy J Mitchison
Date: Mar 18, 2021，Views：1002
Original protocol used in research article：
Co-movement of astral microtubules, organelles and F-actin by dynein and actomyosin forces in frog egg cytoplasm
eLife, Dec 7, 2020; DOI: 10.7554/eLife.60047
Characterization techniques and analyses
Author: Hadi Shafiee
Date: Mar 18, 2021，Views：864
Original protocol used in research article：
Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354
Reduced silver staining
Authors: Gabriella Hannah Wolff and Nicholas James Strausfeld
Date: Mar 18, 2021，Views：868
Original protocol used in research article：
An insect-like mushroom body in a crustacean brain
eLife, Sep 26, 2017; DOI: 10.7554/eLife.29889
Cell Clustering and cluster identification
Author: Michael L Wallace
Date: Mar 18, 2021，Views：937
Original protocol used in research article：
Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271
First and second strand cDNA synthesis and pre-amplification
Author: Arthur M Talman
Date: Mar 18, 2021，Views：929
Original protocol used in research article：
Single-cell RNA-seq reveals hidden transcriptional variation in malaria parasites
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33105
Generation of transgenic lines
Authors: Oriol Llorà-Batlle and Alfred Cortés
Date: Mar 17, 2021，Views：792
Original protocol used in research article：
Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz5057
Isolation of T-cell subpopulations
Authors: Anastasia A Minervina and Mikhail V Pogorelyy
Date: Mar 17, 2021，Views：1376
Original protocol used in research article：
Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
eLife, Feb 21, 2020; DOI: 10.7554/eLife.53704
Strand-specific lncRNA sequencing and analysis
Authors: Shiqi Luo and Xin Zhou
Date: Mar 17, 2021，Views：898
Original protocol used in research article：
Gene reuse facilitates rapid radiation and independent adaptation to diverse habitats in the Asian honeybee
Science Advances, Dec 17, 2020; DOI: 10.1126/sciadv.abd3590
Generate dot surface of the channel hole for CALHM1 using HOLE program
Authors: Xue Yang and Yuequan Shen
Date: Mar 16, 2021，Views：928
Original protocol used in research article：
Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161
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