Protocols shared by authors via RaP service
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Zebrafish retinal dissociation, cell sorting, and methanol fixation
Authors: Patrick Boyd and David R. Hyde
Date: Mar 29, 2021，Views：930
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
Cortical excitatory neuron differentiation
Author: Dina Simkin
Date: Mar 29, 2021，Views：1074
Original protocol used in research article：
Dyshomeostatic modulation of Ca2+-activated K+ channels in a human neuronal model of KCNQ2 encephalopathy
eLife, Feb 5, 2021; DOI: 10.7554/eLife.64434
Parasite strains, culturing and resistance selection
Author: Eva S Istvan
Date: Mar 28, 2021，Views：908
Original protocol used in research article：
Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis
eLife, Mar 19, 2019; DOI: 10.7554/eLife.40529
PIV analysis
Author: HaiYang Wang
Date: Mar 28, 2021，Views：997
Original protocol used in research article：
Symmetry breaking in hydrodynamic forces drives meiotic spindle rotation in mammalian oocytes
Science Advances, Apr 3, 2020; DOI: 10.1126/sciadv.aaz5004
Spinning-disc confocal and 2P3A-DSLM imaging
Authors: Jia Zhao, Yanmei Liu and Liangyi Chen
Date: Mar 27, 2021，Views：1149
Original protocol used in research article：
In vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development
eLife, Jan 29, 2019; DOI: 10.7554/eLife.41540
Phylogenetic analysis of sleep-relevant genes
Authors: Junko Kusumi, Hiroyuki J. Kanaya and Taichi Q. Itoh
Date: Mar 27, 2021，Views：825
Original protocol used in research article：
A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb9415
Ce3D+ and Ce3D++ Tissue Clearing
Authors: Matthew J. Anderson, Valentin Magidson and Mark Lewandoski
Date: Mar 26, 2021，Views：1289
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
DNA binding assays
Author: Kyle W Muir
Date: Mar 26, 2021，Views：906
Original protocol used in research article：
Structural basis for Scc3-dependent cohesin recruitment to chromatin
eLife, Aug 15, 2018; DOI: 10.7554/eLife.38356
Hydroxylamine experiments
Authors: Luca Palazzo, Ivan Matic and Ivan Ahel
Date: Mar 26, 2021，Views：1099
Original protocol used in research article：
Serine is the major residue for ADP-ribosylation upon DNA damage
eLife, Feb 26, 2018; DOI: 10.7554/eLife.34334
Sucrose gradient fractionation
Author: Aleksra Filipovska
Date: Mar 25, 2021，Views：872
Original protocol used in research article：
Fidelity of translation initiation is required for coordinated respiratory complex assembly
Science Advances, Dec 19, 2019; DOI: 10.1126/sciadv.aay2118
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