Protocols shared by authors via RaP service
Journal
Immunofluorescence
Authors: Shang Yin Chiang, Ruey-Hwa Chen and Shen-Ju Chou
Date: Apr 2, 2021，Views：856
Original protocol used in research article：
Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abc6093
Cell staining and imaging
Author: Anne E Carpenter
Date: Apr 2, 2021，Views：952
Original protocol used in research article：
Systematic morphological profiling of human gene and allele function via Cell Painting
eLife, Mar 18, 2017; DOI: 10.7554/eLife.24060
NMR measurements of phase-separating samples
Authors: Rob W. H. Ruigrok and Martin Blackledge
Date: Apr 2, 2021，Views：887
Original protocol used in research article：
Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly
Science Advances, Apr 1, 2020; DOI: 10.1126/sciadv.aaz7095
Calculation of dice scores
Authors: Yunyun Han and Peng Fei
Date: Apr 1, 2021，Views：850
Original protocol used in research article：
Bi-channel image registration and deep-learning segmentation (BIRDS) for efficient, versatile 3D mapping of mouse brain
eLife, Jan 18, 2021; DOI: 10.7554/eLife.63455
Differentiation of human and mouse THGM cells in vitro
Authors: Javad Rasouli, Bogoljub Ciric and Abdolmohamad Rostami
Date: Apr 1, 2021，Views：941
Original protocol used in research article：
A distinct GM-CSF+ T helper cell subset requires T-bet to adopt a TH1 phenotype and promote neuroinflammation
Science Immunology, Oct 22, 2020; DOI: 10.1126/sciimmunol.aba9953
PDC isolation and culture
Authors: Li-Wei Dong, Ye-Xiong Tan and Hong-Yang Wang
Date: Apr 1, 2021，Views：837
Original protocol used in research article：
PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aay0152
Activity recording
Authors: Yusaku Hontani, Fei Xia and Chris Xu
Date: Mar 31, 2021，Views：980
Original protocol used in research article：
Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abf3531
Optogenetic control of CA production
Authors: Meng C Wang and Jeffrey J Tabor
Date: Mar 31, 2021，Views：864
Original protocol used in research article：
Optogenetic control of gut bacterial metabolism to promote longevity
eLife, Dec 16, 2020; DOI: 10.7554/eLife.56849
Preparation of αSyn PFFs
Author: Jin-Song Bian
Date: Mar 30, 2021，Views：904
Original protocol used in research article：
Anti–Na+/K+-ATPase immunotherapy ameliorates α-synuclein pathology through activation of Na+/K+-ATPase α1–dependent autophagy
Science Advances, Jan 26, 2021; DOI: 10.1126/sciadv.abc5062
ND-labeling C. elegans worms
Authors: Masazumi Fujiwara, Eriko Kage-Nakadai and Simo Sun
Date: Mar 30, 2021，Views：831
Original protocol used in research article：
Real-time nanodiamond thermometry probing in vivo thermogenic responses
Science Advances, Sep 11, 2020; DOI: 10.1126/sciadv.aba9636
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