Protocols shared by authors via RaP service
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Analysis of protein stability by synthesis shutoff
Authors: Ira Buntenbroich, Tânia Simões and Mafalda Escobar-Henriques
Date: Mar 19, 2021，Views：1155
Original protocol used in research article：
Cdc48 regulates a deubiquitylase cascade critical for mitochondrial fusion
eLife, Jan 8, 2018; DOI: 10.7554/eLife.30015
Cell culturing and treatments
Author: Massimiliano Clamer
Date: Mar 19, 2021，Views：885
Original protocol used in research article：
One-shot analysis of translated mammalian lncRNAs with AHARIBO
eLife, Feb 17, 2021; DOI: 10.7554/eLife.59303
Protein extraction from sucrose gradient fractions
Author: Massimiliano Clamer
Date: Mar 19, 2021，Views：959
Original protocol used in research article：
One-shot analysis of translated mammalian lncRNAs with AHARIBO
eLife, Feb 17, 2021; DOI: 10.7554/eLife.59303
Bacterial isolation, genome amplification and DNA extraction
Authors: Panagiotis Sapountzis and Panagiotis Sapountzis
Date: Mar 19, 2021，Views：922
Original protocol used in research article：
Reconstructing the functions of endosymbiotic Mollicutes in fungus-growing ants
eLife, Nov 20, 2018; DOI: 10.7554/eLife.39209
Cryo-EM data collection
Authors: Sunbin Deng and Ronen Marmorstein
Date: Mar 18, 2021，Views：821
Original protocol used in research article：
Molecular basis for N-terminal alpha-synuclein acetylation by human NatB
eLife, Sep 4, 2020; DOI: 10.7554/eLife.57491
PIV
Authors: James F Pelletier and Timothy J Mitchison
Date: Mar 18, 2021，Views：995
Original protocol used in research article：
Co-movement of astral microtubules, organelles and F-actin by dynein and actomyosin forces in frog egg cytoplasm
eLife, Dec 7, 2020; DOI: 10.7554/eLife.60047
Characterization techniques and analyses
Author: Hadi Shafiee
Date: Mar 18, 2021，Views：864
Original protocol used in research article：
Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354
Reduced silver staining
Authors: Gabriella Hannah Wolff and Nicholas James Strausfeld
Date: Mar 18, 2021，Views：866
Original protocol used in research article：
An insect-like mushroom body in a crustacean brain
eLife, Sep 26, 2017; DOI: 10.7554/eLife.29889
Cell Clustering and cluster identification
Author: Michael L Wallace
Date: Mar 18, 2021，Views：928
Original protocol used in research article：
Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271
First and second strand cDNA synthesis and pre-amplification
Author: Arthur M Talman
Date: Mar 18, 2021，Views：924
Original protocol used in research article：
Single-cell RNA-seq reveals hidden transcriptional variation in malaria parasites
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33105
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