Protocols shared by authors via RaP service
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Isolation of PDGFRβ+ REPs
Authors: Guangfeng Geng, Quan Chen, Yushan Zhu and Lihong Shi
Date: Aug 15, 2024，Views：118
Original protocol used in research article：
Receptor-mediated mitophagy regulates EPO production and protects against renal anemia
eLife, May 4, 2021; DOI: 10.7554/eLife.64480
In Vivo T Cell Migration Assay
Authors: Francisco Osorio-Barrios and Rodrigo Pacheco
Date: Aug 8, 2024，Views：87
Original protocol used in research article：
The Heteromeric Complex Formed by Dopamine Receptor D5 and CCR9 Leads the Gut Homing of CD4+ T Cells Upon Inflammation
Cellular and Molecular Gastroenterology and Hepatology, Apr 20, 2021; DOI: 10.1016/j.jcmgh.2021.04.006
Primers for validation of Rosa-Gpr56 mice
Authors: Diankun Yu and Xianhua Piao
Date: Aug 1, 2024，Views：94
Original protocol used in research article：
Microglial GPR56 is the molecular target of maternal immune activation-induced parvalbumin-positive interneuron deficits
Science Advances, May 6, 2022; DOI: 10.1126/sciadv.abm2545
Stable expression in HeLa cells and MEFs by retrovirus infection
Authors: Hayashi Yamamoto and Noboru Mizushima
Date: Jul 23, 2024，Views：172
Original protocol used in research article：
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
eLife, ; DOI: 10.7554/eLife.78923
Protease expression and purification
Authors: Celia A Schiffer, Ronald Swanstrom and Ean Spielvogel
Date: Jul 22, 2024，Views：125
Original protocol used in research article：
Selection of HIV-1 for resistance to fifth-generation protease inhibitors reveals two independent pathways to high-level resistance
eLife, Mar 15, 2023; DOI: 10.7554/eLife.80328
Determination of copper concentration in rice by ICP-OES
Authors: Yi Li and Shengze Yao
Date: Jul 21, 2024，Views：86
Original protocol used in research article：
The key micronutrient copper orchestrates broad-spectrum virus resistance in rice
Science Advances, Jul 1, 2022; DOI: 10.1126/sciadv.abm0660
Neuronal cultures
Authors: Guiping Wang, Marc Tessier-Lavigne and Xiaowei Zhuang
Date: Jul 17, 2024，Views：88
Original protocol used in research article：
Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration
eLife, May 1, 2019; DOI: 10.7554/eLife.38730
Prediction of functional impact of variants using PROVEAN
Authors: Stefano G Giulieri, Timothy P Stinear and Benjamin P Howden
Date: Jul 14, 2024，Views：92
Original protocol used in research article：
Niche-specific genome degradation and convergent evolution shaping Staphylococcus aureus adaptation during severe infections
eLife, ; DOI: 10.7554/eLife.77195
Sample preparation for FLAG pull down
Authors: Donghai Wang, Hudan Liu, Kaiwei Liang and Guoliang Qing
Date: Jul 10, 2024，Views：104
Original protocol used in research article：
The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastoma
Science Advances, Mar 29, 2023; DOI: 10.1126/sciadv.adf0005
Tonoplast membrane preparation
Authors: Upendo Lupanga and Karin Schumacher
Date: Jul 9, 2024，Views：85
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
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