Bio-101 - Improve Research Reproducibility

RNA pull-down assay

Authors: Chuannan Fan and Peter ten Dijke
Date: Jun 13, 2024，Views：15

Original protocol used in research article：

The lncRNA LETS1 promotes TGF-β–induced EMT and cancer cell migration by transcriptionally activating a TβR1-stabilizing mechanism
SCIENCE SIGNALING, Jun 20, 2023; DOI: 10.1126/scisignal.adf1947

Intracytoplasmic sperm injection（ICSI）

Authors: Yongliang Shang, Fei Gao, Xuejiang Guo, Qing-Yuan Sun, Yunxia Cao and Wei Li
Date: Jun 7, 2024，Views：375

Original protocol used in research article：

Essential role for SUN5 in anchoring sperm head to the tail
eLife, Sep 25, 2017; DOI: 10.7554/eLife.28199

Biochemical measurement of Rab5 activity

Author: Muralidharan Mani
Date: Jun 4, 2024，Views：283

Original protocol used in research article：

Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling
Molecular Biology of the Cell, Jan 15, 2016; DOI: 10.1091/mbc.E15-08-0558

RNA isolation, cDNA synthesis, and quantitative RT-PCR

Authors: Keshav Thapa and Petteri Rinne
Date: Jun 4, 2024，Views：617

Original protocol used in research article：

Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver
eLife, Jul 25, 2023; DOI: 10.7554/eLife.84782

Mouse intestinal epithelial cell strip

Authors: Timothy J Nice and David A Constant
Date: May 24, 2024，Views：548

Original protocol used in research article：

Homeostatic interferon-lambda response to bacterial microbiota stimulates preemptive antiviral defense within discrete pockets of intestinal epithelium
eLife, Feb 9, 2022; DOI: 10.7554/eLife.74072

Ex vivo muscle function testing

Authors: Jeffrey Molendijk, Ronnie Blazev and Benjamin L Parker
Date: May 14, 2024，Views：500

Original protocol used in research article：

Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function
eLife, Dec 6, 2022; DOI: 10.7554/eLife.82951

Histology and immunofluorescence staining and analysis

Author: Derek C Sung
Date: May 13, 2024，Views：531

Original protocol used in research article：

VE-cadherin enables trophoblast endovascular invasion and spiral artery remodeling during placental development
eLife, Apr 29, 2022; DOI: 10.7554/eLife.77241

Culture of naïve and primed hPSCs

Author: Thorold W Theunissen
Date: May 8, 2024，Views：23

Original protocol used in research article：

Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504

Plasmids preparation and lentiviral production

Authors: Albert H. Kim, Diane Mao and Rowland Han
Date: May 8, 2024，Views：584

Original protocol used in research article：

TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma
Science Advances, May 12, 2023; DOI: 10.1126/sciadv.ade3559

4.2. Arabidopsis Genotypes Growth Conditions and Salt Stress Induction

Authors: Nkulu Kabange Rolly and Byung-Wook Yun
Date: May 7, 2024，Views：268

Original protocol used in research article：

Salinity Stress-Mediated Suppression of Expression of Salt Overly Sensitive Signaling Pathway Genes Suggests Negative Regulation by AtbZIP62 Transcription Factor in Arabidopsis thaliana
International Journal of Molecular Sciences, Mar 3, 2020; DOI: 10.3390/ijms21051726

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