Protocols shared by authors via RaP service
Journal
Mice
Author: Bin Zhou
Date: Jun 3, 2021，Views：881
Original protocol used in research article：
Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair
Science, Feb 26, 2021; DOI: 10.1126/science.abc4346
Culturing splenic, hepatic and alveolar macrophages
Author: Alexandre A Steiner
Date: Jun 2, 2021，Views：840
Original protocol used in research article：
A leukotriene-dependent spleen-liver axis drives TNF production in systemic inflammation
Science Signaling, Apr 20, 2021; DOI: 10.1126/scisignal.abb0969
Study design
Authors: Adam Yala and Regina Barzilay
Date: Jun 1, 2021，Views：933
Original protocol used in research article：
Toward robust mammography-based models for breast cancer risk
Science Translational Medicine, Jan 27, 2021; DOI: 10.1126/scitranslmed.aba4373
Statistical analysis
Author: Adam Yala
Date: Jun 1, 2021，Views：895
Original protocol used in research article：
Toward robust mammography-based models for breast cancer risk
Science Translational Medicine, Jan 27, 2021; DOI: 10.1126/scitranslmed.aba4373
T cell isolation
Authors: Hasan Zaki, Yang-Xin Fu and Chuanhui Han
Date: Jun 1, 2021，Views：1005
Original protocol used in research article：
The AIM2 and NLRP3 inflammasomes trigger IL-1–mediated antitumor effects during radiation
Science Immunology, May 6, 2021; DOI: 10.1126/sciimmunol.abc6998
Immune memory assessment
Authors: Kai Yang and Zhuang Liu
Date: Jun 1, 2021，Views：897
Original protocol used in research article：
Bacteria-triggered tumor-specific thrombosis to enable potent photothermal immunotherapy of cancer
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.aba3546
Confocal microscopy and image analysis
Authors: Jan N Hansen, Fabian Kaiser, Florian I Schmidt and Dagmar Wachten
Date: Jun 1, 2021，Views：1038
Original protocol used in research article：
Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium
eLife, Jun 24, 2020; DOI: 10.7554/eLife.57907
PCR amplification and next generation sequencing of sgRNAs
Authors: Xiangbing Qi and Ting Han
Date: May 31, 2021，Views：1097
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Data analysis for genome-wide CRISPR knockout screening
Authors: Xiangbing Qi and Ting Han
Date: May 31, 2021，Views：953
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Co-immunoprecipitation of CCNK and DDB1 with 3xFLAG-CDK12 complex
Authors: Longzhi Cao, Xiangbing Qi and Ting Han
Date: May 31, 2021，Views：985
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
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