Protocols shared by authors via RaP service
Journal
Sorting of antigen-specific single B cells by flow cytometry
Author: Wei Jiang
Date: Jun 23, 2021，Views：824
Original protocol used in research article：
Systemic translocation of Staphylococcus drives autoantibody production in HIV disease
Microbiome, Feb 14, 2019; DOI: 10.1186/s40168-019-0646-1
Serum-free primary astrocyte culture
Author: Raymundo D Hernandez
Date: Jun 23, 2021，Views：760
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
BV2 Microglial Culture and Microglial Exosome Isolation
Author: Yusheng Wang
Date: Jun 23, 2021，Views：931
Original protocol used in research article：
Exosomes from Microglia Attenuate Photoreceptor Injury and Neovascularization in an Animal Model of Retinopathy of Prematurity
Molecular Therapy. Nucleic Acids, May 17, 2019; DOI: 10.1016/j.omtn.2019.04.029
SILAC labeling and DNA pull-down experiments
Authors: Reuben S Harris and Dennis Kappei
Date: Jun 23, 2021，Views：912
Original protocol used in research article：
Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
eLife, Sep 28, 2020; DOI: 10.7554/eLife.61287
In vitro BBB penetration of NPs
Authors: Nitin Joshi and Sezin Aday
Date: Jun 23, 2021，Views：1053
Original protocol used in research article：
BBB pathophysiology–independent delivery of siRNA in traumatic brain injury
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd6889
2.7. In Vivo Wounding Assay
Author: Vladimir Krylov
Date: Jun 23, 2021，Views：799
Original protocol used in research article：
Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells
Stem Cells International, May 5, 2019; DOI: 10.1155/2019/8387478
ATG5 siRNA Transfection
Author: Anna-Mart Engelbrecht
Date: Jun 23, 2021，Views：836
Original protocol used in research article：
Amino Acid Starvation Sensitizes Resistant Breast Cancer to Doxorubicin-Induced Cell Death
Frontiers in Cell and Developmental Biology, Oct 15, 2020; DOI: 10.3389/fcell.2020.565915
CRISPR/Cas9 PRMT1 knockout
Authors: María F. Montenegro and José Neptuno Rodríguez-López
Date: Jun 23, 2021，Views：825
Original protocol used in research article：
PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation
Scientific Reports, Jan 1, 2020; DOI: 10.1038/s41598-020-70289-3
Multistep resistance generation evaluation
Authors: Silei Bai, Jianxue Wang, Yugang Bai and Xinxin Feng
Date: Jun 22, 2021，Views：862
Original protocol used in research article：
A polymeric approach toward resistance-resistant antimicrobial agent with dual-selective mechanisms of action
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abc9917
Assays of cellular stress.
Authors: Olumuyiwa Igbalajobi, Zhenzhong Yu and Reinhard Fischer
Date: Jun 22, 2021，Views：816
Original protocol used in research article：
Red- and Blue-Light Sensing in the Plant Pathogen Alternaria alternata Depends on Phytochrome and the White-Collar Protein LreA
mBio, Apr 9, 2019; DOI: 10.1128/mBio.00371-19
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