Protocols shared by authors via RaP service
Journal
Xenograft in zebrafish
Author: Ji Eun Lee
Date: Jun 19, 2021，Views：820
Original protocol used in research article：
CEP41‐mediated ciliary tubulin glutamylation drives angiogenesis through AURKA‐dependent deciliation
EMBO Reports, Dec 29, 2019; DOI: 10.15252/embr.201948290
Expression and purification of recombinant SARS-CoV-2 Spike protein
Authors: Jun Zhang, Yongfei Cai and Bing Chen
Date: Jun 18, 2021，Views：1130
Original protocol used in research article：
Structural impact on SARS-CoV-2 spike protein by D614G substitution
Science, Apr 30, 2021; DOI: 10.1126/science.abf2303
Glutamate clearance assay.
Authors: Venkata Viswanadh Edara and Kathleen Borgmann
Date: Jun 18, 2021，Views：807
Original protocol used in research article：
Insights into the Gene Expression Profiles of Active and Restricted Red/Green-HIV+ Human Astrocytes: Implications for Shock or Lock Therapies in the Brain
Journal of Virology, Feb 28, 2020; DOI: 10.1128/JVI.01563-19
Social Interaction Test
Authors: Cassandra M. Moshfegh and Adam J Case
Date: Jun 17, 2021，Views：1305
Original protocol used in research article：
Autonomic and Redox Imbalance Correlates With T-Lymphocyte Inflammation in a Model of Chronic Social Defeat Stress
Frontiers in Behavioral Neuroscience, May 14, 2019; DOI: 10.3389/fnbeh.2019.00103
Loading and release of siRNA from albumin NPs
Authors: Maria G. Castro and Joerg Lahann
Date: Jun 17, 2021，Views：861
Original protocol used in research article：
Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy
Nature Communications, Nov 10, 2020; DOI: 10.1038/s41467-020-19225-7
Heterotopic Cardiac Transplantation
Authors: Reza Motallebzadeh and Gavin J. Pettigrew
Date: Jun 17, 2021，Views：891
Original protocol used in research article：
Relative Frequencies of Alloantigen-Specific Helper CD4 T Cells and B Cells Determine Mode of Antibody-Mediated Allograft Rejection
Frontiers in Immunology, Jan 22, 2019; DOI: 10.3389/fimmu.2018.03039
Establishment of PD-1 Flag-tagged constructs
Authors: Judith Weyershaeuser and Maja Köhn
Date: Jun 16, 2021，Views：870
Original protocol used in research article：
Molecular mechanism of SHP2 activation by PD-1 stimulation
Science Advances, Jan 30, 2020; DOI: 10.1126/sciadv.aay4458
In vitro co-culture and 3D matrigel/collagen culture assays
Author: Don L. Gibbons
Date: Jun 15, 2021，Views：848
Original protocol used in research article：
Collagen promotes anti-PD-1/PD-L1 resistance in cancer through LAIR1-dependent CD8+ T cell exhaustion
Nature Communications, Sep 9, 2020; DOI: 10.1038/s41467-020-18298-8
In vitro microtubule-binding assays
Authors: Jianguo Chen and Junlin Teng
Date: Jun 12, 2021，Views：836
Original protocol used in research article：
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment
BMC Biology, Sep 14, 2019; DOI: 10.1186/s12915-019-0694-9
Complex I Enzymatic Activity Assay
Author: Shaobai Huang
Date: Jun 11, 2021，Views：820
Original protocol used in research article：
Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes1,[OPEN]
Plant Physiology, Jun 22, 2020; DOI: 10.1104/pp.20.00136
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