Protocols shared by authors via RaP service
Journal
Wounding Zebrafish Larval Epidermis by Laceration
Authors: Andrew S Kennard, Ellen Labuz, Christopher K Prinz and Julie A Theriot
Date: Jun 3, 2021，Views：1175
Original protocol used in research article：
Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis
eLife, Nov 23, 2020; DOI: 10.7554/eLife.62386
Real-Time PCR
Authors: Ming Xu and James L Kirkland
Date: Jun 3, 2021，Views：999
Original protocol used in research article：
Targeting senescent cells enhances adipogenesis and metabolic function in old age
eLife, Dec 19, 2015; DOI: 10.7554/eLife.12997
Reagents and antibodies
Author: Hongyu Deng
Date: Jun 3, 2021，Views：839
Original protocol used in research article：
PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion
Science Advances, Nov 19, 2020; DOI: 10.1126/sciadv.abd0276
Histone modification ChIP-seq on Arabidopsis thaliana plantlets
Authors: Juan Sebastian Ramirez Prado, David Latrasse and Moussa Benhamed
Date: Jun 3, 2021，Views：1253
Original protocol used in research article：
A new role for histone demethylases in the maintenance of plant genome integrity
eLife, Oct 27, 2020; DOI: 10.7554/eLife.58533
Mice
Author: Bin Zhou
Date: Jun 3, 2021，Views：880
Original protocol used in research article：
Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair
Science, Feb 26, 2021; DOI: 10.1126/science.abc4346
Culturing splenic, hepatic and alveolar macrophages
Author: Alexandre A Steiner
Date: Jun 2, 2021，Views：840
Original protocol used in research article：
A leukotriene-dependent spleen-liver axis drives TNF production in systemic inflammation
Science Signaling, Apr 20, 2021; DOI: 10.1126/scisignal.abb0969
Study design
Authors: Adam Yala and Regina Barzilay
Date: Jun 1, 2021，Views：932
Original protocol used in research article：
Toward robust mammography-based models for breast cancer risk
Science Translational Medicine, Jan 27, 2021; DOI: 10.1126/scitranslmed.aba4373
Statistical analysis
Author: Adam Yala
Date: Jun 1, 2021，Views：893
Original protocol used in research article：
Toward robust mammography-based models for breast cancer risk
Science Translational Medicine, Jan 27, 2021; DOI: 10.1126/scitranslmed.aba4373
T cell isolation
Authors: Hasan Zaki, Yang-Xin Fu and Chuanhui Han
Date: Jun 1, 2021，Views：1004
Original protocol used in research article：
The AIM2 and NLRP3 inflammasomes trigger IL-1–mediated antitumor effects during radiation
Science Immunology, May 6, 2021; DOI: 10.1126/sciimmunol.abc6998
Immune memory assessment
Authors: Kai Yang and Zhuang Liu
Date: Jun 1, 2021，Views：897
Original protocol used in research article：
Bacteria-triggered tumor-specific thrombosis to enable potent photothermal immunotherapy of cancer
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.aba3546
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