Protocols shared by authors via RaP service
Journal
Whole-mount immunostaining
Authors: Caroline Lei Wee, Sam Kunes and Erin Song
Date: May 26, 2021，Views：1057
Original protocol used in research article：
A bidirectional network for appetite control in larval zebrafish
eLife, Oct 18, 2019; DOI: 10.7554/eLife.43775
Fecundity assays
Authors: Brooke Zanco and Matthew DW Piper
Date: May 26, 2021，Views：1643
Original protocol used in research article：
A dietary sterol trade-off determines lifespan responses to dietary restriction in Drosophila melanogaster females
eLife, Jan 26, 2021; DOI: 10.7554/eLife.62335
scRNA/TCR-seq data processing
Authors: Paul Gueguen, Joshua J. Waterfall and Sebastian Amigorena
Date: May 26, 2021，Views：972
Original protocol used in research article：
Contribution of resident and circulating precursors to tumor-infiltrating CD8+ T cell populations in lung cancer
Science Immunology, Jan 29, 2021; DOI: 10.1126/sciimmunol.abd5778
Generation of cell lines stably expressing ACVR1-IPF
Authors: Anassuya Ramachandran and Caroline S Hill
Date: May 25, 2021，Views：823
Original protocol used in research article：
TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition
eLife, Jan 29, 2018; DOI: 10.7554/eLife.31756
Flow cytometry analysis of the CAR-transduced cells
Authors: Vanaja Konduri and William K. Decker
Date: May 25, 2021，Views：965
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
Preparation of DCs
Authors: Emilie V Russler-Germain and Chyi-Song Hsieh
Date: May 25, 2021，Views：963
Original protocol used in research article：
Gut Helicobacter presentation by multiple dendritic cell subsets enables context-specific regulatory T cell generation
eLife, Feb 3, 2021; DOI: 10.7554/eLife.54792
Immunocytochemistry
Authors: Y. Zheng, G. Zhao and J. Fu
Date: May 24, 2021，Views：858
Original protocol used in research article：
Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI: 10.1126/sciadv.aax5933
Cell preparation and flow cytometry
Author: Niklas K. Björkström
Date: May 24, 2021，Views：926
Original protocol used in research article：
Natural killer cell immunotypes related to COVID-19 disease severity
Science Immunology, Aug 21, 2020; DOI: 10.1126/sciimmunol.abd6832
Histology and Immunohistochemistry
Authors: Yiqiao Hu, Jinhui Wu and Huanhuan Chen
Date: May 23, 2021，Views：912
Original protocol used in research article：
Dissolved oxygen from microalgae-gel patch promotes chronic wound healing in diabetes
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aba4311
Fabrication of ZIF-8, NV-ZIF, and NV-ZIFMCF
Author: Niveen M. Khashab
Date: May 23, 2021，Views：1031
Original protocol used in research article：
Sustained and targeted delivery of checkpoint inhibitors by metal-organic frameworks for cancer immunotherapy
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abe7174
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