Protocols shared by authors via RaP service
Journal
In vitro Infection of Neutrophils With Leishmania donovani Promastigotes
Authors: Mareike Ohms, Tamás Laskay and Sonja Möller
Date: Jun 27, 2021，Views：767
Original protocol used in research article：
An Attempt to Polarize Human Neutrophils Toward N1 and N2 Phenotypes in vitro
Frontiers in Immunology, Apr 28, 2020; DOI: 10.3389/fimmu.2020.00532
In vitro SIRT6 deacetylation assay
Authors: Minxian Qian and Baohua Liu
Date: Jun 27, 2021，Views：882
Original protocol used in research article：
Deacetylase-independent function of SIRT6 couples GATA4 transcription factor and epigenetic activation against cardiomyocyte apoptosis
Nucleic Acids Research, Apr 2, 2020; DOI: 10.1093/nar/gkaa214
In utero electroporation of mouse embryos
Author: Wieland B Huttner
Date: Jun 26, 2021，Views：1012
Original protocol used in research article：
Extracellular matrix-inducing Sox9 promotes both basal progenitor proliferation and gliogenesis in developing neocortex
eLife, Mar 19, 2020; DOI: 10.7554/eLife.49808
Cell-free BoDV preparation.
Authors: Keizo Tomonaga and Akiko Makino
Date: Jun 25, 2021，Views：781
Original protocol used in research article：
ADAR2 Is Involved in Self and Nonself Recognition of Borna Disease Virus Genomic RNA in the Nucleus
Journal of Virology, Feb 28, 2020; DOI: 10.1128/JVI.01513-19
AAV vector production
Authors: Sean K Wang and Constance L. Cepko
Date: Jun 25, 2021，Views：900
Original protocol used in research article：
Microglia modulation by TGF-β1 protects cones in mouse models of retinal degeneration
The Journal of Clinical Investigation, Jul 13, 2020; DOI: 10.1172/JCI136160
Primary neuron-astrocyte co-culture
Authors: Michelle L Olsen and Raymundo D Hernandez
Date: Jun 25, 2021，Views：869
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
Generation of In vitro Derived Mucus
Authors: Lisa H. Cazares and Sonja Hess
Date: Jun 25, 2021，Views：782
Original protocol used in research article：
Development of a Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of SARS-CoV-2 Spike Glycoprotein and Nucleoprotein
Anal Chem, Sep 23, 2020; DOI: 10.1021/acs.analchem.0c02288
Linearity of the assay
Author: Nicolas Chomont
Date: Jun 25, 2021，Views：770
Original protocol used in research article：
Single-cell characterization and quantification of translation-competent viral reservoirs in treated and untreated HIV infection
PLoS Pathogens, Feb 27, 2019; DOI: 10.1371/journal.ppat.1007619
Isolation, culture and flow cytometry of bone marrow B cell progenitors.
Authors: Malay Mandal and Marcus R Clark
Date: Jun 24, 2021，Views：735
Original protocol used in research article：
CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis
Nat Immunol, Sep 2, 2019; DOI: 10.1038/s41590-019-0468-0
FRET-based nucleosomes sliding
Authors: Robert L. Yauch and Tharu Fernando
Date: Jun 24, 2021，Views：749
Original protocol used in research article：
Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patients
Nature Communications, Nov 3, 2020; DOI: 10.1038/s41467-020-19402-8
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