Protocols shared by authors via RaP service
Journal
Site-directed mutagenesis and vector construction
Author: Qing-Lin Liu
Date: Nov 7, 2021，Views：718
Original protocol used in research article：
Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature
BMC Genomics, Jan 14, 2021; DOI: 10.1186/s12864-020-07365-5
Updated protocols for measurement of GSIS and calcium imaging of islet clusters
Authors: Jia Zhao, Shenghui Liang, Guang Gao, Liangyi Chen and Yanmei Liu
Date: Oct 31, 2021，Views：2229
Original protocol used in research article：
In vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development
eLife, Jan 29, 2019; DOI: 10.7554/eLife.41540
Purification of recombinant bacterial proteins
Authors: Min Young Kim and Chul Geun Kim
Date: Oct 31, 2021，Views：841
Original protocol used in research article：
Rational discovery of antimetastatic agents targeting the intrinsically disordered region of MBD2
Science Advances, Nov 19, 2019; DOI: 10.1126/sciadv.aav9810
Environmental Scanning Electron Microscopy (SEM)
Authors: Lucie Riglet, Isabelle Fobis-Loisy and Thierry Gaude
Date: Oct 14, 2021，Views：944
Original protocol used in research article：
KATANIN-dependent mechanical properties of the stigmatic cell wall mediate the pollen tube path in Arabidopsis
eLife, Sep 1, 2020; DOI: 10.7554/eLife.57282
MALDI-MS analysis
Authors: Yan Zhang, Joshua E Mayfield and Seema Irani
Date: Oct 11, 2021，Views：826
Original protocol used in research article：
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
eLife, Aug 6, 2019; DOI: 10.7554/eLife.48725
Manufacturing of hOBMT
Authors: Nathalie Bock, Dietmar W. Hutmacher and Judith A. Clements
Date: Oct 10, 2021，Views：861
Original protocol used in research article：
In vitro engineering of a bone metastases model allows for study of the effects of antiandrogen therapies in advanced prostate cancer
Science Advances, Jun 30, 2021; DOI: 10.1126/sciadv.abg2564
Multifunctionality assessments
Authors: Marcel G. A. van der Heijden and Raphaël Albert Wittwer
Date: Oct 6, 2021，Views：818
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
PARP1 Purification Protocol
Authors: Pam Dyer, Johannes Rudolph and Karolin Luger
Date: Sep 29, 2021，Views：958
Original protocol used in research article：
Poly(ADP-ribose) polymerase 1 searches DNA via a ‘monkey bar’ mechanism
eLife, Aug 8, 2018; DOI: 10.7554/eLife.37818
Headplate and cranial window surgery for two-photon imaging of inferior colliculus in transgenic mice
Authors: Aaron Benson Wong and J Gerard G Borst
Date: Sep 28, 2021，Views：962
Original protocol used in research article：
Tonotopic and non-auditory organization of the mouse dorsal inferior colliculus revealed by two-photon imaging
eLife, Oct 15, 2019; DOI: 10.7554/eLife.49091
Generation of transgenic lines
Authors: Wenqing Zhang and Jin Xu
Date: Sep 13, 2021，Views：887
Original protocol used in research article：
Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells
eLife, Jun 15, 2018; DOI: 10.7554/eLife.36131
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