Protocols shared by authors via RaP service
Journal
RNA-Seq
Authors: Prashant Rajbhandari and Peter Tontonoz
Date: Feb 4, 2022，Views：791
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Antibodies
Authors: Ji Eun Oh and Akiko Iwasaki
Date: Feb 2, 2022，Views：730
Original protocol used in research article：
Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA
Science Immunology, Dec 10, 2021; DOI: 10.1126/sciimmunol.abj5129
Enzyme-linked immunosorbent assay
Authors: Ji Eun Oh and Akiko Iwasaki
Date: Feb 2, 2022，Views：834
Original protocol used in research article：
Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA
Science Immunology, Dec 10, 2021; DOI: 10.1126/sciimmunol.abj5129
Computational-driven epitope verification and affinity maturation of TLR4-targeting antibodies
Author: Bilal Ahmad
Date: Feb 2, 2022，Views：732
Original protocol used in research article：
Computational-Driven Epitope Verification and Affinity Maturation of TLR4-Targeting Antibodies
International Journal of Molecular Sciences, Jun 1, 2021; DOI: 10.3390/ijms22115989
Tumor model generation and treatment
Authors: Leila Akkari and Johanna A. Joyce
Date: Feb 2, 2022，Views：763
Original protocol used in research article：
Dynamic changes in glioma macrophage populations after radiotherapy reveal CSF-1R inhibition as a strategy to overcome resistance
Science Translational Medicine, Jul 14, 2020; DOI: 10.1126/scitranslmed.aaw7843
Library construction and single-clone sequencing
Authors: Chong Zhang and Ming Shao
Date: Feb 1, 2022，Views：735
Original protocol used in research article：
Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish
Science Advances, Aug 5, 2021; DOI: 10.1126/sciadv.abg4243
ES and goods calculation
Authors: Raphaël A. Wittwer and Marcel G. A. van der Heijden
Date: Feb 1, 2022，Views：822
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
Data collection
Authors: Raphaël A. Wittwer and Marcel G. A. van der Heijden
Date: Feb 1, 2022，Views：746
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
Statistical analyses
Authors: Raphaël A. Wittwer and Marcel G. A. van der Heijden
Date: Feb 1, 2022，Views：730
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
Surgery and injection of viral vector
Authors: Christopher R Fetsch and Michael N Shadlen
Date: Jan 30, 2022，Views：781
Original protocol used in research article：
Focal optogenetic suppression in macaque area MT biases direction discrimination and decision confidence, but only transiently
eLife, Jul 27, 2018; DOI: 10.7554/eLife.36523
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