Bio-101 - Improve Research Reproducibility

Structural variants

Author: Kamil S. Jaron
Date: Mar 8, 2022，Views：595

Original protocol used in research article：

Convergent consequences of parthenogenesis on stick insect genomes
Science Advances, Feb 23, 2022; DOI: 10.1126/sciadv.abg3842

Translatome purification with RiboTag

Authors: Walker S. Jackson and Lech Tadeusz Kaczmarczyk
Date: Mar 8, 2022，Views：601

Original protocol used in research article：

Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background
Scientific Reports, Mar 8, 2021; DOI: 10.1038/s41598-021-84887-2

Primary osteoclast culture

Authors: Eben G Estell and Clifford J Rosen
Date: Mar 7, 2022，Views：937

Original protocol used in research article：

Irisin directly stimulates osteoclastogenesis and bone resorption in vitro and in vivo
eLife, Aug 11, 2020; DOI: 10.7554/eLife.58172

Larvae dissociation

Authors: Maria Ina Arnone and Periklis Paganos
Date: Mar 7, 2022，Views：674

Original protocol used in research article：

Single-cell RNA sequencing of the Strongylocentrotus purpuratus larva reveals the blueprint of major cell types and nervous system of a non-chordate deuterostome
eLife, Nov 25, 2021; DOI: 10.7554/eLife.70416

Animal models

Authors: I-Lin Ho and Andrea Viale
Date: Mar 7, 2022，Views：637

Original protocol used in research article：

Epithelial memory of inflammation limits tissue damage while promoting pancreatic tumorigenesis
Science, Sep 17, 2021; DOI: 10.1126/science.abj0486

Senescence-associated β-galactosidase assay

Authors: Mounir El Maï and Miguel Godinho Ferreira
Date: Mar 7, 2022，Views：899

Original protocol used in research article：

Opposing p53 and mTOR/AKT promote an in vivo switch from apoptosis to senescence upon telomere shortening in zebrafish
eLife, May 19, 2020; DOI: 10.7554/eLife.54935

Crown delineation

Author: Ben G Weinstein
Date: Mar 7, 2022，Views：684

Original protocol used in research article：

A remote sensing derived data set of 100 million individual tree crowns for the National Ecological Observatory Network
eLife, Feb 19, 2021; DOI: 10.7554/eLife.62922

2.2.2. Maceration Extraction (ME)

Authors: Jurga Andreja Kazlauskaite and Jurga Bernatoniene
Date: Mar 6, 2022，Views：590

Original protocol used in research article：

Novel Extraction Method Using Excipients to Enhance Yield of Genistein and Daidzein in Trifolium pratensis L.
Pharmaceutics, May 22, 2021; DOI: 10.3390/pharmaceutics13060777

Drug preparation

Author: Lu Chen
Date: Mar 6, 2022，Views：734

Original protocol used in research article：

Defective memory engram reactivation underlies impaired fear memory recall in Fragile X syndrome
eLife, Nov 20, 2020; DOI: 10.7554/eLife.61882

Phytaspase cleavage assay using synthetic oligopeptide substrates

Authors: A. Stintzi and A. Schaller
Date: Mar 4, 2022，Views：654

Original protocol used in research article：

Peptide signaling for drought-induced tomato flower drop
Science, Mar 26, 2020; DOI: 10.1126/science.aaz5641

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