Protocols shared by authors via RaP service
Journal
Induction of rEpiLCs and rPGCLCs
Authors: Mami Oikawa, Masumi Hirabayashi and Toshihiro Kobayashi
Date: Apr 19, 2022，Views：627
Original protocol used in research article：
Functional primordial germ cell–like cells from pluripotent stem cells in rats
Science, Apr 7, 2022; DOI: 10.1126/science.abl4412
scRNA-seq data processing
Authors: Wei Xie, Xiaotong Wu and Hongmei Zhang
Date: Apr 18, 2022，Views：617
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Checkerboard Method
Authors: Ping Huang and Yifei Xu
Date: Apr 18, 2022，Views：633
Original protocol used in research article：
Unraveling the Novel Effect of Patchouli Alcohol Against the Antibiotic Resistance of Helicobacter pylori
Frontiers in Microbiology, Jun 2, 2021; DOI: 10.3389/fmicb.2021.674560
Mouse models
Authors: Xiaofei Chen, Xiao Chen and Jiacan Su
Date: Apr 18, 2022，Views：565
Original protocol used in research article：
Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abb7135
In vitro phosphorylation assay and LC-MS/MS
Authors: Lin Wang, Noe Perron, Bowen Tan, Craig P. Dufresne, Sixue chen and Xiuren Zhang
Date: Apr 14, 2022，Views：678
Original protocol used in research article：
PRP4KA phosphorylates SERRATE for degradation via 20S proteasome to fine-tune miRNA production in Arabidopsis
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm8435
In vitro polysaccharide precipitation assays and fungal protection assays against chitinases
Authors: Ioannis Stergiopoulos and Li-Hung Chen
Date: Apr 13, 2022，Views：608
Original protocol used in research article：
A diverse member of the fungal Avr4 effector family interacts with de-esterified pectin in plant cell walls to disrupt their integrity
Science Advances, May 7, 2021; DOI: 10.1126/sciadv.abe0809
Protein analysis
Author: Shadmehr Demehri
Date: Apr 13, 2022，Views：640
Original protocol used in research article：
CD8+ T cell immunity blocks the metastasis of carcinogen-exposed breast cancer
Science Advances, Jun 17, 2021; DOI: 10.1126/sciadv.abd8936
Clustering of PVH neuron functional responses measured by CaRMA imaging
Authors: Shengjin Xu, Hui Yang and Scott Sternson
Date: Apr 11, 2022，Views：647
Original protocol used in research article：
Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI: 10.1126/science.abb2494
Methods
Authors: Jiacan Su, Mingyao Liu and Jian Luo
Date: Apr 11, 2022，Views：590
Original protocol used in research article：
Regulation of body length and bone mass by Gpr126/Adgrg6
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aaz0368
Comparative metabolic profiling
Authors: Jin-Ming Yang and Jianxun Song
Date: Apr 8, 2022，Views：582
Original protocol used in research article：
Elongation factor-2 kinase is a critical determinant of the fate and antitumor immunity of CD8+ T cells
Science Advances, Feb 2, 2022; DOI: 10.1126/sciadv.abl9783
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