Protocols shared by authors via RaP service
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Neutral comet assay
Author: Dongyi Xu
Date: Sep 19, 2020，Views：900
Original protocol used in research article：
53BP1 and BRCA1 control pathway choice for stalled replication restart
eLife, Nov 6, 2017; DOI: 10.7554/eLife.30523
S1 nuclease fiber assay
Authors: Sumeet Nayak and Sharon B. Cantor
Date: Sep 18, 2020，Views：1690
Original protocol used in research article：
Inhibition of the translesion synthesis polymerase REV1 exploits replication gaps as a cancer vulnerability
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz7808
Skin-penetrating IL-RNA complexes
Author: Abhirup Mandal
Date: Sep 18, 2020，Views：1000
Original protocol used in research article：
Treatment of psoriasis with NFKBIZ siRNA using topical ionic liquid formulations
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.abb6049
Statistics
Author: Carlos Guijas
Date: Sep 18, 2020，Views：850
Original protocol used in research article：
Metabolic adaptation to calorie restriction
Science Signaling, Sep 8, 2020; DOI: 10.1126/scisignal.abb2490
Immunostaining of fish scales
Author: Valerie Wittamer
Date: Sep 18, 2020，Views：907
Original protocol used in research article：
Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes
eLife, May 5, 2020; DOI: 10.7554/eLife.53403
Immunofluorescence
Authors: Ruihua Ma and Beatriz Sosa-Pineda
Date: Sep 17, 2020，Views：1087
Original protocol used in research article：
Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts
eLife, Mar 10, 2020; DOI: 10.7554/eLife.46206
RNAscope in situ hybridization
Author: Glenda Comai
Date: Sep 16, 2020，Views：1871
Original protocol used in research article：
A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
eLife, Sep 19, 2019; DOI: 10.7554/eLife.47460
Flow cytometry antibody panel
Authors: Alexandre Boissonnas and Pierre Louis Loyher
Date: Sep 16, 2020，Views：988
Original protocol used in research article：
Immune surveillance of the lung by migrating tissue monocytes
eLife, Jul 13, 2015; DOI: 10.7554/eLife.07847
Cryo–electron tomography
Authors: M. A. Benhaim, K. K. Lee and Vidya Mangala Prasad
Date: Sep 15, 2020，Views：837
Original protocol used in research article：
Structural monitoring of a transient intermediate in the hemagglutinin fusion machinery on influenza virions
Science Advances, Apr 28, 2020; DOI: 10.1126/sciadv.aaz8822
Exosome isolation, Subcellular fractionation, and TCA precipitation
Author: Karoliina Stefanius
Date: Sep 14, 2020，Views：1172
Original protocol used in research article：
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
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